中间锦鸡儿转录因子基因 CiNAC1的克隆及功能分析
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内蒙古自治区高等学校科学研究项目(NJZY055);


Cloning and Functional Analysis of CiNAC1 from Caragana intermedia
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    摘要:

    NAC转录因子家族是植物特有的、最大的转录因子家族之一,在植物应答非生物胁迫和生长发育过程中有重要的功能。该研究通过PCR技术,克隆得到了中间锦鸡儿 CiNAC1基因1 066 bp的cDNA全长序列。生物信息学分析显示, CiNAC1基因的开放阅读框(ORF)为921 bp,编码306个氨基酸,推导的蛋白分子量为34.57 kD,等电点为8.35,是一种亲水性蛋白,N端具有保守的NAM结构域,具有26个磷酸化位点和7个糖基化位点。实时荧光定量PCR检测显示,中间锦鸡儿 CiNAC1基因表达受干旱、高盐、脱水、高pH诱导;亚细胞定位发现CiNAC1定位到细胞核中,这与它作为转录因子的功能是一致的;转CiNAC1基因拟南芥株系侧根数目显著多于野生型,根长也明显比野生型长。研究认为, CiNAC1基因可能与中间锦鸡儿响应逆境胁迫机制有关。

    Abstract:

    NACs are one of the largest plantspecific transcription factor families. Members in this family play important roles in growth and abiotic stress responses. In this study, the fulllength cDNA sequence of CiNAC1 from Caragana intermedia was cloned by PCR technique. It was 1 066 bp in length. Bioinformatics analysis showed that CiNAC1 had an open reading frame of 921 bp, encoding 306 amino acids. The calculated molecular weight of CiNAC1 was 34.57 kD and the isoionic point was 8.35. The CiNAC1 protein was a hydrophilic protein which had a conserved NAM domain in the Nterminus. It contained 26 phosphorylation sites and 7 glycosylation sites. Realtime quantitative PCR analysis showed that CiNAC1 was induced by drought, NaCl, dehydration, and high pH. Localization assays revealed that CiNAC1 localized in the nuclei, consistent with its role as transcription factor. Overexpression of CiNAC1 promoted the lateral root formation and length of the primary root. These results indicated that CiNAC1 might be related to response to environmental stress in C. intermedia.

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岳俊燕,岳文冉,杨 杞,等.中间锦鸡儿转录因子基因 CiNAC1的克隆及功能分析[J].西北植物学报,2016,36(7):1285-1293

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  • 在线发布日期: 2016-08-16
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