NtMTP1基因过表达对增强烟草Zn胁迫耐受性的研究
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国家自然科学基金(31500205);


Overexpression of NtMTP1 Gene from Nicotiana tabacum Improved Zinc Tolerance of Tobacco
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    摘要:

    该研究采用实时荧光定量PCR(qRTPCR)技术,对烟草金属耐受蛋白1(MTP1)基因(NtMTP1)在烟草不同组织以及不同质量浓度ZnSO4处理下的表达进行了分析;利用农杆菌介导法,将NtMTP1基因植物过表达载体pBI12135S∶∶MTP1转化野生型烟草,筛选得到NtMTP1基因过表达的转基因烟草植株,并进行不同质量浓度ZnSO4处理,检测NtMTP1基因过表达对烟草Zn胁迫耐受性的影响。结果表明:NtMTP1基因在烟草中呈现组织特异性表达,主要在花与叶中表达;NtMTP1基因的表达受到Zn2+诱导,在400 μmol/L ZnSO4处理后,表达量达到最高,为对照组的3.81倍;3株转基因烟草植株中NtMTP1基因表达量分别为野生型的10.42、7.61和11.84倍,与野生型相比,过表达植株对Zn胁迫的耐受性显著增强。研究结果为阐明NtMTP1基因在烟草体内Zn2+转运过程中的生物学功能提供了重要依据。

    Abstract:

    In this study, the relative expression of NtMTP1 was determined by quantitative realtime PCR (qRTPCR). The results showed that NtMTP1 gene was highly expressed in flowers and leaves. After treatment of 400 μmol/L ZnSO4 for 48 h, the expression of NtMTP1 increased to the maximum, which was 3.81 folds of the control. Plantexpression vector pBI12135S∶∶MTP1 was constructed, and the recombinant plasmid was introduced into Nicotiana tabacum L. cv NC89 by means of Agrobacteriumtumefaciens mediated transformation. The qRTPCR revealed a distinct increase of endogenous NtMTP1 expression level in the overexpressing transgenic lines, which were 10.42, 7.61 and 11.84 folds of that of wild plants, respectively. Seeds of wild type and transgenic plants were treated with different concentrations of ZnSO4. The results showed that overexpression of NtMTP1 conferred enhanced zinc tolerance. These results provide an important basis to illuminate the function of NtMTP1 gene in Zn2+ transport in tobacco.

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冉 巧,吴婵娟,高永峰,等.NtMTP1基因过表达对增强烟草Zn胁迫耐受性的研究[J].西北植物学报,2016,36(7):1308-1314

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  • 在线发布日期: 2016-08-16
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