Abstract:To explore the phosphatesolubilizing ability of endophytic fungi and their plant growthpromoting potential, we evaluated the phosphatesolubilizing capability of endophytic fungi from ancient Sophora japonica leaves in Zhougongmiao by the method of dissolved phosphorus cycle and molybdenumbule, and detected the effects of inoculated sterile host plantlet on chlorophyll, soluble protein contents, SOD and POD activities. The results showed that, there were 28 out of 55 fungi strains from ancient S. japonica leaves with phosphatesolubilizing capability, and twelve strains among twentyeight endophytic fungi displayed strong capability of dissolving phosphate of Ca3(PO4)2 by a test of phosphatesolubilizing capability, they were Pestalotiopsis sp.(ZG7), Fusarium sp.(ZG9, ZG23, ZG32, ZG36 and ZG53), Aspergillus sp.(ZG15 and ZG34), Alternaria sp.(ZG39 and ZG51), Trichoderma sp.(ZG42) and Epicoccum sp.(ZG48). Two strains with strong phosphatesolubilizing capability were then picked up, ZG15 and ZG34, belonged to Aspergillus spp..Their diameter of phosphorus solubilizing circle (d) and d/D (D, colony diameter) were both higher than that of others,and their solubilization of Ca3(PO4)2 accounted for 1 238.28 mg·L-1 and 941.22 mg·L-1, respectively, which were significantly higher than that of others and control (P<0.01). Four strains (ZG7, ZG9, ZG15 and ZG48) from different genera were inoculated into aseptic S. japonica seedlings bottle to ensure their contact upon with each other. Mycelium was observed infecting into the cortex cells and around the surface epidemical cells of root tissue; under the treatment by Aspergillus niger ZG15, S. japonica seedlings chorophyll content, soluble protein content, SOD and POD net activities were all obviously higher than that of control (P<0.05), so, ZG15 could maintain healthy seedling growth and improve seedling resilience, which showed a great potential for ancient S. japonica protection and a potential application as an organismfertilizer for forest tree.