Abstract:The expression profiles of 5 AGO genes(AGO1,AGO2,AGO4,AGO10 and AGO18)in different developmental stages of maize seed(Zea mays L.inbred line,‘Chang 72’)after pollination were analyzed by the method of realtime quantitative PCR.Results showed that, the expression pattern of AGO1 and AGO2 were similar during maize seed development, showing a decline trend from the 7th day after pollination (DAP) to the 20th DAP, with much more accumulation at the 7th DAP.The expression pattern of AGO4, AGO10 and AGO18 were similar, with a trend of being decreased first then increased, with the lowest expression at the 10th DAP.With our previous results of expression pattern of miRNAs during maize seed development, we found that different AGO gene families together with their target miRNAs, can involved in the regulation of maize seed development.