We isolated and identified an organophosphatesolubilizing bacterium JYD4 from the rhizosphere of _{Taxus chinensis} var. _mairei. Molybdenumantimony antispectrophotometric method was applied to investigate the effects of carbon sources, nitrogen sources and pH environments on organophosphate solubilization capacity. We also detected the effects of temperatures and pH on the phosphatase enzyme activity produced by strain JYD4. The inoculation test in potted seedlings was carried out under the greenhouse. The results showed that: (1) strain JYD4 was identified as _{Stenotrophomonas maltophilia}. (2) The ratio of organophosphatesolubilizing circle diameter to colony diameter was 2.01. Organophosphatedissolving ability in liquid medium was 72.38 mg/L. (3) Glucose and beef extract were identified as the optimal carbon source and nitrogen source for organophosphatesolubilizing. The optimal pH was 7.0. (4) Phosphatase produced by _{S. maltophilia} JYD4 mainly located in the cell. The phosphatase could maintain stable activity in a wide temperature range (2065 ℃), only conferred high activity at pH 9.0. (5) _{S. maltophilia} JYD4 was able to improve the seedling height, ground diameter and biomass of the seedlings of _{T. chinensis} var. _mairei. The results showed that _{S. maltophilia} JYD4 is a highly effective organophosphatesolubilizing bacterium. It could significantly promote the growth of _{Taxus chinensis} var. _mairei. Results in this paper provide excellent strain for the development of microbial fertilizer of _{T. chinensis} var. _mairei, and provide theory evidences for the application of S. maltophilia JYD4.