Abstract:Using RTPCR technique, we cloned a gibberellin receptor gene from cormels of Gladiolus hybridus named as GhGID1a (genbank accession number KU525107). The open reading frame of GhGID1a was 1 032 bp, which encodes 343 amino acids. The sequence alignment results indicated that the deduced amino acid sequence of GhGID1a was similar to the GID amino acid sequences of Agapanthus praecox,Elaeis guineensis and Phoenix dactylifera,with the similarity of 82%, 78% and 77%, respectively. 50 mg/L GA3 could promote cormel germination, but GA3 at 150 mg/L could inhibit germination. Realtime PCR results indicated that GA3 treatments caused feedbackinhibition on GhGID1a expression. The GhGID1a expression gradually decreased with cormel dormancy release, which inferred that GA and its receptor gene GhGID1a participated in the regulation process of Gladiolus hybridus cormel dormancy and germination.