水分胁迫下不同抗旱性小麦品种叶片转录因子表达差异研究
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西北农林科技大学青年学术骨干研究基金(01140302)


Comparative Expression of Two Functionknown Transcription Genes in Different Drought Tolerance Wheat Cultivars under Water Deficit Stress
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    摘要:

    通过研究不同抗旱性小麦品种中转录因子表达水平的差异,为阐明小麦抗旱机制奠定基础。依据候选基因序列设计PCR引物,以干旱胁迫后0、3、6、9、12和24 h的小麦叶片为实验材料,以26S rRNA为内参,运用荧光定量PCR技术,检测Wdreb2、Wlip19基因在干旱敏感性和干旱耐受性小麦叶片中的相对表达量。定量PCR结果显示:干旱胁迫后,Wdreb2、Wlip19基因在干旱敏感性小麦叶片中的表达明显低于干旱耐受性小麦,在不同品种叶片中的响应时间和表达趋势存在差异。研究认为,Wdreb2、Wlip19基因在不同品种小麦受到干旱胁迫后的表达差异,与该品种小麦的抗旱能力具有一定的相关性。

    Abstract:

    This study aimed to investigate the expression of Wdreb2 and Wlip19 in ten different varieties of wheat leaves under drought stress, and lay a foundation for wheat drought resistance mechanism. Primers were designed according to gene sequences, using wheat leaves under different degrees of drought stress (including 0,3,6,9,12 and 24 h) as experimental materials, and 26S rRNA as the internal control. The florescent realtime quantitative PCR was used to detect relative expression levels of Wdreb2 and Wlip19 in different varieties of wheat. RTPCR results showed that, the expression of Wdreb2 and Wlip19 gene in drought sensitivity wheat leaf significantly lower than that in drought tolerant wheat under drought stress. There are some differences in response time and express trends in different varieties. These results indicated that the different expression levels of Wdreb2 and Wlip19 in different varieties of wheat under drought stress were related to the drought resistant ability.

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秦 鹏,刘秉焱,韩翠英,等.水分胁迫下不同抗旱性小麦品种叶片转录因子表达差异研究[J].西北植物学报,2016,36(11):2267-2272

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  • 在线发布日期: 2016-12-12
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