拟南芥砷诱导基因At4g13180的表达模式研究
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国家自然科学基金(31570247, 91417308, 31460453);


Expression Pattern Analysis of Arabidopsis thaliana Arsenic Induced Gene At4g13180
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    拟南芥(Arabidopsis thaliana)砷诱导基因At4g13180编码蛋白是短链脱氢酶(ShortChain Dehydrogenase/Reductase Superfamily,SDR)家族的成员之一,其过表达可以增强植物对过氧化氢的耐受性。该实验通过半定量RTPCR,构建ProAt4g13180:GUSAt4g13180EGFP和At4g13180OE表达载体,获得At4g13180基因过表达转基因株系,并研究了At4g13180基因的表达模式及其编码蛋白的亚细胞定位。结果显示,At4g13180基因在根尖、叶脉、萼片和花丝等组织都强烈表达,该基因编码蛋白主要定位于胞质和核中。该研究结果为深入探究拟南芥砷诱导基因At4g13180的功能奠定了一定的基础。

    Abstract:

    In Arabidopsis thaliana, the arsenic induced gene At4g13180 encoding protein is one of the members of the SDR superfamily (ShortChain Dehydrogenase/Reductase). Its overexpression can enhance the resistance of plants to hydrogen peroxide. Here we studied the At4g13180 gene expression pattern and the encoded protein subcellular localization by semiquantitative RTPCR, with transgenic plants expressing ProAt4g13180:GUS, At4g13180EGFP and At4g13180OE. Our results show that the At4g13180 gene has a very strong expression in tissues such as root, leaf, sepal, and stamen filamentas. At4g13180EGFP is located in the nucleus and cytoplasm. Our study lays a basis of further research on the role of the arsenic induced gene At4g13180 in A. thaliana.

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魏情情,包曙光,门淑珍.拟南芥砷诱导基因At4g13180的表达模式研究[J].西北植物学报,2016,36(12):2349-2353

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  • 在线发布日期: 2017-01-20
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