SlCBL1基因在番茄抗灰霉病中的作用
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江苏省自然科学基金(BK20160567);


The Role of SlCBL1 Gene in the Resistance to Botrytis cinerea of Tomato
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    摘要:

    以番茄(Solanum lycopersicum L.)品种‘Micro Tom’为试材,分析番茄叶片和果实的灰霉病发病规律,及番茄类钙调磷酸酶B基因(tomato calcineurin Blike gene,SlCBL1)在叶片和果实中的表达变化;比较转SlCBL1基因番茄与对照的叶片和果实的灰霉病发病过程,分析转基因番茄抗病相关转录因子表达变化。结果表明:(1)非转基因番茄中,不同叶龄的叶片均在接种灰霉病4 d开始发病;不同发育阶段的果实接种灰霉病后发病时间也不同,其中绿果(花后16~18 d)接种5 d还未发病,白果(花后34~36 d)接种11 d开始发病,红果(花后40~42 d)接种5 d开始发病;SlCBL1基因表达量在番茄叶片中较低,在绿果期和白果期的果实中表达量最高,红果期果实中表达量最低。(2)转SlCBL1基因后,SlCBL1基因的过量表达能够抑制番茄叶片和果实灰霉病发生;同时番茄叶片和果实中几乎所有的抗病转录因子的表达量都上调,其中WRKY转录因子家族基因SlWRKY33和 SlWRKY70受到强烈调控。研究说明,SlCBL1基因过量表达能够提高番茄的抗灰霉能力,其主要机理是通过影响抗病相关转录因子进而调控番茄抗灰霉病的能力。

    Abstract:

    With tomato (Solanum lycopersicum L.) variety ‘Micro Tom’ as test materials, we analyzed the pathogenesis regularity and SlCBL1 gene expression in tomato leaf and fruit. After inoculation with Botrytis cinerea, we observed the incidence of SlCBL1 gene in tomato leaves and fruits in transgenic tomato and control. We analyzed the expression change of transcription factor related to disease resistance in transgenic tomato and control. The results show that: (1) for Nongmo tomatoes, the leaves began to attack after inoculation B. cinerea 4 days. Green fruit inoculation B. cinerea 15 days still no disease. White fruit began to attack after inoculation B. cinerea 11 days. Red fruit began to attack after inoculation B. cinerea 5 days. SlCBL1 gene expression in tomato leaves was lower than in fruit, green fruit and white fruit stage is the highest, the red fruit is the lowest. (2) For genetically modified tomatoes, overexpression of SlCBL1 gene can inhibit the occurrence of B. cinerea in tomato leaves and fruits. Almost all the transcription factor expression was upregulated in leaves and fruits. WRKY transcription factor family gene SlWRKY33 and SlWRKY70 were strongly upregulated. The results showed that the over expression of SlCBL1 gene could improve the ability of the tomato to resist the B. cinerea. And by influencing the disease resistance related transcription factors to regulation tomato resistance to B. cinerea.

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王媛花,颜志明,解振强,等.SlCBL1基因在番茄抗灰霉病中的作用[J].西北植物学报,2016,36(12):2376-2384

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  • 在线发布日期: 2017-01-20
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