Abstract:With tomato (Solanum lycopersicum L.) variety ‘Micro Tom’ as test materials, we analyzed the pathogenesis regularity and SlCBL1 gene expression in tomato leaf and fruit. After inoculation with Botrytis cinerea, we observed the incidence of SlCBL1 gene in tomato leaves and fruits in transgenic tomato and control. We analyzed the expression change of transcription factor related to disease resistance in transgenic tomato and control. The results show that: (1) for Nongmo tomatoes, the leaves began to attack after inoculation B. cinerea 4 days. Green fruit inoculation B. cinerea 15 days still no disease. White fruit began to attack after inoculation B. cinerea 11 days. Red fruit began to attack after inoculation B. cinerea 5 days. SlCBL1 gene expression in tomato leaves was lower than in fruit, green fruit and white fruit stage is the highest, the red fruit is the lowest. (2) For genetically modified tomatoes, overexpression of SlCBL1 gene can inhibit the occurrence of B. cinerea in tomato leaves and fruits. Almost all the transcription factor expression was upregulated in leaves and fruits. WRKY transcription factor family gene SlWRKY33 and SlWRKY70 were strongly upregulated. The results showed that the over expression of SlCBL1 gene could improve the ability of the tomato to resist the B. cinerea. And by influencing the disease resistance related transcription factors to regulation tomato resistance to B. cinerea.