Abstract:In order to study the biosynthesis of sesquiterpene, this text analyzed the sesquiterpene synthetases gene(AcTPS2) through genome analysis of Antrodia camphorata, and obtained the full length cDNA with RTPCR, carried on bioinformatics and mRNA analysis. The results showed that AcTPS2 cDNA has 1 086 bp; Blast analysis revealed that AcTPS2 included abundant asparagic acid motif DXXXD that is specific motif of sesquiterpene synthetase, and terpene synthetase special conserved sequence RRDTSGLDL; phylogenetic trees showed AcTPS2 and other fungus sesquiterpene synthetase amino acid sequence clustered in one clade; mRNA analysis showed manitol as carbon source, casein peptone as nitrogen source could promote AcTPS2 effectively, and the results could supply some basis in sesquiterpene biosynthesis.