In order to explore the effects of temperature on pollen tube growth and protective enzyme activities of early maturing loquat in the 1st florescence, we took the 1st florescence of a new very precocious loquat line ‘Chuanzao loquat’ as experimental material to study the stigma receptivity, pollen vitality, pollen tube growth and protective enzyme activities under three temperature treatments of T₁ (25 ℃/20 ℃, day/night), T₂ (30 ℃/25 ℃, day/night) and T₃ (35 ℃/30 ℃, day/night). The results showed that: (1) the duration of stigma with receptivity, the pollen vitality under the same flowers’ age and the number of pollen tubes reached the style base of the 1st florescence of ‘Chuanzao loquat’ were ranked as T₁ > T₂ > T₃, and the length of time of pollen tubes arrived at style base was ranked as T₁ >T₃＞T₂. The pollen tubes growth showed apex bending, swelling, stop growth and other anomalies in the style at T₃. (2) The SOD and CAT activities of flowers in three temperature treatments presented the trends of first increased then decreased with the increase of flowers’ age, but POD activity presented the trends of first decreased then increased. The protective enzyme activities maintained at a relatively stable level at T₁, but had the significant changes at T₂ and T₃. In conclusion, the optimal temperature is 25 ℃/20 ℃, day/night for fruiting in the 1st florescence, change the amplitude increased of main antioxidant enzyme activity, the short duration of stigma receptivity, low pollen vitality and pollen tube growth abnormalities in style under high temperature resulted the bad fruit setting rate of early maturing loquat in the 1st florescence.