蒙古冰草Lhcb1基因克隆及干旱胁迫下的表达分析
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国家自然科学基金(31260578,31160479);


Cloning and Expression Analysis of Lhcb1 from Agropyron mongolicum Keng under Drought Stress
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    摘要:

    利用同源序列从蒙古冰草(Agropyron mongolicum Keng)克隆得到1个光合作用叶绿体结合a/b基因,命名为MwLhcb1。MwLhcb1基因cDNA全长1 138 bp,包含801 bp开放阅读框,编码267个氨基酸,蛋白分子量为28.21 kD,等电点4.92。该蛋白二级结构中具有Lhcb基因家族的保守结构域。MwLhcb1蛋白氨基酸序列与其他物种同类蛋白相似性均在87%以上,其中与小麦同类蛋白相似性程度最高达99%。实时荧光定量PCR结果显示,MwLhcb1基因主要在茎叶中表达,在根中表达量极少,干旱胁迫影响MwLhcb1基因表达。该研究结果为进一步研究MwLHcb1在蒙古冰草光合作用与抗旱性中的功能奠定了基础,并从基因遗传进化角度证实了蒙古冰草是小麦野生近缘种的观点,从而提出蒙古冰草是小麦抗性改良的理想基因供体。

    Abstract:

    The gene of lightharvesting cholorophyll a/bbinding proteins was cloned by homologous sequences from Agropyron mongolicum Keng, named MwLhcb1. The fulllength of cDNA sequence was 1 138 bp,bioinformatics analysis showed that MwLhcb1 had an open reading frame of 801 bp, encoding 267 amino acids. The calculated molecular weight of MwLhcb1 was 28.21 kD and isoelectric point was 4.92. The MwLhcb1 protein had a conserved domain of Lhcb gene family in the secondary structure. Phylogenetic analysis revealed that MwLhcb1 amino acids sequences was highly similar with the homologous proteins of other species, it was more than 87%, and the similarity degree of protein with wheat was the highest, it is 99%. Realtime quantitative PCR analysis showed that MwLhcb1 gene was mainly expressed in stem and leaf, and the expression in the root was minimal. The expression of MwLhcb1 gene was affected by drought stress, and the expression of MwLhcb1 gene was different under the different levels of drought stress. The results could be a foundation for further study on the function of MwLhcb1 gene in photosynthesis and drought resistance of A. mongolicum. Meanwhile, it confirmed was wild relatives of wheat based on gene genetic and evolution, so we supported that was ideal donor for improving to wheat resistance.

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赵 彦,高 鑫,王 丹,等.蒙古冰草Lhcb1基因克隆及干旱胁迫下的表达分析[J].西北植物学报,2017,37(2):211-216

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  • 在线发布日期: 2017-03-23
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