Abstract:WRKY transcription factors play an important adjusting role in the process of plant growth and development, hormone signal transduction and abiotic stress response. To clarify the gene Narcissus tazetea var. ‘Yunxiang’, we cloned the NtWRKYY1 gene (GenBank accession No. KX056495) based on ‘Yunxiang’, analyzed the gene sequence features, evolutionary relationship and expression characteristics, constructed the expression vector before transformed into tobacco. Sequence analysis revealed that the length of NtWRKYY1 gene open reading frame (ORF) is 510 bp, encoding a polypeptide of 169 amino acids. Multiple alignments and phylogenetic analysis showed that NtWRKYY1 protein containing one WRKY consecutive structural domain and C2H2 type zinc finger(Cx4Cx23HxH)belong to Ⅱc subgroup of WRKY transcription factor together with Arabidopsis AtWRKY57. Tissuespecific expression and temporal and spatial expression showed that NtWRKYY1 in ‘Yunxiang’ had a much higher expression in flowers than that in roots and leaves, and a rising express trend in petal and corona of alabastrum stage, early flowering stage, full bloom stage and faded stage. NtWRKYY1 can be induced by abscisic acid (ABA), high temperature, drought and saline, and restrained by methyl jasmonate (JA) through hormones and abiotic stresses analyzing. We can concluded that NtWRKYY1 gene may play a regulating role during the flower senescence process in ‘Yunxiang’, involve in the hormone signal transduction of ABA, JA and the abiotic stress regulation of high temperature, drought and saline at the same time. In addition, we constructed overexpressing vector pMDC140NtWRKYY1 using InFusion cloning technique, transformed into tobacco by the method of Agrobacterium though leaf disc transformation. The carrier of PCR and GUS staining results indicated the resistant plantlets were positive. This study will make a good foundation for further exploring the function of the WRKY transcription factor in ‘Yunxiang’.