DREB transcription factor belongs to the family of AP2/ERF transcription factors, which can specifically bind to DRE/CRT cisacting elements, regulate the expression of stress response genes and play an important role in stress response to low temperature, drought and high salt stress. The members of DREB transcription factor family were identified by bioinformatics analysis, and the conserved domain of DREB transcription factor family was analyzed by using the whole genome data of apple, and the function and expression of DREB transcription factor family were analyzed. The results showed that 60 members of DREB transcription factor family were identified from the whole genome of apple, which was consistent with that of Arabidopsis and rice. The phylogenetic analysis of Arabidopsis DREB gene could be further subdivided into 6 Subgroups; Domains and conserved elements analysis showed that the DREB gene family contained an AP2 conserved domain, chromosomal loci indicated that the DREB gene of apple was distributed on 11 chromosomes, and some genes had tandem duplication. Analysis of the gene structure showed that the subfamily gene did not contain introns. The analysis of RNASeq data of Arabidopsis thaliana showed that the DREB transcription factor family regulates abiotic stress such as low temperature and ABA regulation, and each subgroup in the DREB subfamily responds to different abiotic stresses. The expression of DREB gene in different tissues was analyzed. The results showed that the expression of DREB gene was the strongest in plant root, followed by leaf.