Abstract:This paper is to study the regulation of accumulation of phenolics compound and the dynamic change law of related enzyme activities in different parts of shoots during development of Xanthoceras sorbifolia Bunge. We analyzed the content of polyphenols, flavonoids, tannins and the activities of related enzymes (phenylalanine ammonialyase polyphenol oxidase) in shoot tips, stems and leaves during the shoots’ growth and development of X. sorbifolia by using shoots. The results showed that: (1) the contents of polyphenols, flavonoids and tannins presented a rising tendency with seasons in development of X. sorbifolia shoots; the contents of polyphenols, flavonoids and tannin were showing the following tendency: leaf>stem tip>stem. (2) The PAL activity of the development of X. sorbifolia new shoots is: young stem segments and corresponding leaves> the semilignified stem segments and corresponding leaves> the lignified stems and corresponding leaves. The PPO activity of leaves was significantly higher than that of stem segments, and the PPO activity of stem apex was the lowest. Leaves of X. sorbifolia had a strong antioxidant capacity; the highest scavenging rate of DPPH· was 98.13%. (3) Before and after April 29th, there was a significant difference in the rising trend of polyphenols, flavonoids and tannins contents; PAL activity of all organs was the lowest and PPO activity was the highest; the DPPH· scavenging rate of stem segments with different degrees of lignification was reduced to the lowest; therefore, April 29th was an important transitional growth stage of the shoot growth period. (4) Correlation analysis of X. sorbifolia new shoots revealed that there was a significant positive correlation between the contents of polyphenols, flavonoids and tannins; there was also a significant positive correlation between the contents of polyphenols, flavonoids, tannins and the scavenging capacities of DPPH·; there was also a positive correlation between the contents of polyphenols, flavonoids, tannins and PPO activity; however, there was not a positive correlation between the contents of polyphenols, flavonoids, tannins and PAL activity. The study suggests that the content of phenolics compound is the main factor affecting the vegetative propagation of X. sorbifolia; the lower the content of phenolics compound is, the higher the survival rate of cuttings, grafting and tissue culture. The content of phenolics compound such as polyphenols, flavonoids, and tannins is the lowest at the early stage of shoot growth, but the content of phenolics compound such as polyphenols, flavonoids and tannins increase significantly with the growth of new shoots. The vegetative propagation of X. sorbifolia is most suitable in the initial stage of shoots growth which the best time is from April to midMay.