Sucrose phosphate synthase (SPS) is a major ratelimiting enzyme to sucrose synthesis in plant, which affects the growth of plants and sucrose content of fruits. To know the features of SPS family gene and their role in sucrose synthesis, we isolated MdSPS family genes from apple genome, analyzed the relationship between their evolutionary relationships, profiled the mRNA expression characteristics, measured the enzyme activity and sucrose content. The results showed that: (1) there were 8 genes of the SPS gene family expressing in the apple, they belong to three dicot SPS subfamilies. (2) Quantitative real time PCR analysis showed that C type MdSPS6 and A type MdSPS1a/b had the highest expression in apple. Especially, MdSPS6 expression levels are the highest in mature fruit, followed by those in mature leaves, meanwhile MdSPS1a/b was primarily expressed in young fruit which had no sucrose accumulation. (3) Apart from MdSPS1a/b, expression levels of other 5 MdSPS family genes had a significant positive correlation with SPS activity and sucrose concentration, and increased with fruit development. These results suggest that the class C of the SPS gene family is main genes related with sucrose syntheses in the development of apple leaves and fruits.