八棱海棠上11个矮化砧木生长及其叶片解剖特性的研究
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甘肃省农牧厅生物技术专项(035034070);


Growth and Leaf Anatomical Structure of 11 Dwarf Rootstocks on Malus robusta Rehd.
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    摘要:

    以八棱海棠(Malus robusta Rehd.)为基砧,在其上嫁接11个矮化砧木(M9、M7、SH38、T337、SH6、SH1、JM7、GM256、‘辽砧2号’、M26、B9),测定嫁接成活率、萌芽率、植株生长指标、叶片解剖参数,并对矮化砧木各测定指标进行相关分析、主成分分析和聚类分析,明确各矮化砧木与八棱海棠的嫁接亲和性,研究11个矮化砧在八棱海棠上的生长特性与叶片解剖结构,比较不同矮化砧木的矮化效应。结果表明:(1)11个矮化砧与八棱海棠均亲和,且以M26、SH38的亲和性最好。(2)嫁接植株的矮化性与年生长量、节间长度、粗度比、叶片上下表皮厚度比、叶片气孔密度和海绵组织厚度均呈负相关关系,而与叶片长宽比、栅海比、叶片厚度、栅栏组织厚度均呈正相关关系。(3)主成分分析提取的4个主成分反映了原变量91.573%的信息,第1主成分(PC1)综合了叶厚、海绵组织厚度、栅栏组织厚度的信息,第2主成分(PC2)综合了叶长宽比、节间长度、年生长量的信息,第3主成分(PC3)综合了叶栅海比、粗度比的信息,第4主成分(PC4)综合了叶气孔密度的信息。(4)根据主成分分析结果,11个矮化砧木可以聚为4类,第1类包括SH1、SH38、SH6、M7、M26,第2类包括B9、JM7,第3类包括T337、M9、GM256,第4类仅包括‘辽砧2号’。研究认为,11个苹果矮化砧与基砧八棱海棠均具有较好的亲和性,它们的矮化性强弱依次为‘辽砧2号’ > GM256 > M9 > T337 > JM7 > B9 > M26 > M7 > SH6 > SH38 >SH1;植株的年生长量、节间长度、接口上下粗度比以及叶片的气孔密度、海绵组织厚度、栅栏组织厚度、栅海比、叶厚度可做为评价矮化性的有效指标。

    Abstract:

    The experiment took Malus robusta Rehd. as rootstock, on which grafting M9, M7, SH38, T337, SH6, SH1, JM7, GM256, ‘Liaozhen 2’, M26, B9. To analyze the correlation among different indexes by determining grafting survival rate, germination rate, plant growth, leaf anatomical data, we studied these 11 dwarf rootstocks by principal component analysis(PCA) and hierarchical cluster analysis(HCA). To learn grafting affinity of different dwarf interstocks with M. robusta Rehd and evaluate their dwarfing traits, we investigated the growth characteristics and leaf anatomical structure of 11 dwarf rootstocks on M. robusta Rehd.. The results showed that: (1) 11 dwarf rootstocks had affinity with M. robusta Rehd, which M26 and SH38 had the strongest affinity. (2) The correlation analysis showed that dwarfing of plant have a negative relatoin with plant height, length of plant internode, diameter ratio, the ratio of upper epidermis thickness, stoma density, thickness of spongy tissue, and its positive with leaf length to width ratio, palisade tissue to spongy tissue ratio, thickness of leaf, thickness of palisade tissue. (3) PCA got four principal components and represented 91.573% of the total variability. The PC1 was related to thickness of leaf, thickness of spongy tissue, thickness of palisade tissue, and the PC2 included length to width ratio, related to plant height, length of plant internode, and the PC3 consisted of palisade tissue to spongy tissue ratio, diameter ratio, and the PC4 was stoma density. (4) According to the result of PCA, 11 dwarf rootstocks were divied ino four types by HCA. The first types includes SH1, SH38, SH6, M7, M26 and the second covers B9, JM7, The third class contains T337, M9, GM256 and the fouth is ‘Liaozhen 2’, which was consistent with the results of PCA. As a result, 11 apple dwarf rootstocks had a better affinity with M. robusta Rehd., which the ranking of dwarf was: ‘Liaozhen 2’ > GM256 > M9 > T337 > JM7 > B9 > M26 > M7 > SH6 > SH38 >SH1.The plant of dwarfing was estimated by plant height, length of plant internode, diameter ratio, the ratio of upper epidermis thickness, stoma density of leaf, thickness of spongy tissue, thickness of palisade tissue, palisade tissue to spongy tissue ratio and thickness of leaf.

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贾旭梅,王延秀,党兆霞,等.八棱海棠上11个矮化砧木生长及其叶片解剖特性的研究[J].西北植物学报,2017,37(6):1126-1136

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  • 在线发布日期: 2017-07-17
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