盐胁迫下芙蓉菊与4种菊属植物生理响应特征及其耐盐机理分析
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北京林业大学青年教师科学研究中长期项目(2015ZCQYL03);


Analysis of Physiological Response and Salt Tolerance Mechanism of Crossostephium chinense and Four Species of Chrysanthemum under Salt Stress
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    摘要:

    以耐盐优异种质芙蓉菊和2种菊属野生种质野菊(天堂寨)、野菊(神农架),以及2种菊属栽培品种‘繁花似锦’和‘寒露红’为材料,用200 mmol·L-1NaCl的1/2 Hoagland营养液进行盐胁迫处理,在盐胁迫第0、5、10、15天分别测定各材料的相关生理指标,比较各材料随盐胁迫时间增加而产生的生理响应特征,探讨芙蓉菊的耐盐生理生化机制。结果显示:随着盐胁迫时间的增加,5个材料的叶绿素含量(Chl)、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、过氧化物酶(POD)活性均呈下降趋势,但芙蓉菊下降不显著或变化趋势与其他4个材料有明显区别;同期各材料的丙二醛(MDA)含量、脯氨酸含量、超氧化物歧化酶(SOD)活性均呈上升趋势,且芙蓉菊的丙二醛含量上升幅度最小;同期胞间CO2浓度在各种质中的变化趋势各异。研究发现,芙蓉菊在盐胁迫下能够保持叶绿素含量和膜系统的相对稳定,从而维持正常水平的净光合速率,这可能是芙蓉菊耐盐性强于其他菊属植物的主要原因。

    Abstract:

    The salt tolerant germplasm Crossostephium chinense and the two wild species of Chrysanthemum, Chrysanthemum indicum (Tiantangzhai) and (Shennongjia), along with two cultivars of Chrysanthemum (‘Fanhuasijin’ and ‘Hanluhong’) were treated with 1/2 Hoagland nutrient solution containing 200 mmol·L-1 NaCl and their physiological response that changed with the increase of saltstress time were compared. The related physiological indexes of each material were measured at 0, 5, 10 and 15 days after salt stress and the physiological responses of each material were compared, in order to preliminarily explore the physiological salttolerance mechanism of C.chinense. The results showed that the contents of chlorophyll (Chl), net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) and the peroxidase activity (POD) in five species decreased with the increase of saltstress time, while C. chinense had the minimum decrease or a significantly different changing trend from other 4 Chrysanthemum species. In the meantime, the content of malondialdehyde (MDA), proline and the activity of superoxide dismutase (SOD) increased with the time, and among them the MDA of C. chinense increased least. The changing trend of intercellular CO2 concentration (Ci) varied in different germplasms. The study has shown that the chlorophyll content and the membrane system were relatively stable under salt stress, and the net photosynthetic rate maintained at a certain level, which may be the main reasons that the salt tolerance of C. chinense was stronger than that of other Chrysanthemum species.

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林双冀, 孙 明.盐胁迫下芙蓉菊与4种菊属植物生理响应特征及其耐盐机理分析[J].西北植物学报,2017,37(6):1137-1144

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  • 在线发布日期: 2017-07-17
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