氮源对杜氏盐藻生长及光合系统Ⅱ的影响
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甘肃省科技支撑计划社会发展类项目(1604FKC090);


Effect of Nitrogen on Growth and PhotosystemⅡ of Dunaliella salina
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    摘要:

    以NaNO3、CO(NH2)2、NaNO2、NH4Cl作为氮源,并设无氮处理,研究杜氏盐藻(Dunaliella salina)生长及其PSⅡ对不同氮源的响应特征,以明确杜氏盐藻对不同氮源的利用情况,为盐藻培养基的优化和营养盐的筛选提供理论依据。结果表明:(1)杜氏盐藻在CO(NH2)2环境中具更高的比增长速率[μmax,(0.482±0.032)/d]。(2)NaNO2、NaNO3、CO(NH2)2作氮源时,杜氏盐藻快速光响应曲线的初始斜率(α)、最大光量子产率(Fv/Fm)及反应活性中心所捕获的光能(ABS/RC)、反应活性中心捕获的激发能用于还原QA的能量(TR0/RC)、最大光化学效率(φP0)、t=0时捕获的激子将电子传递到电子传递链中超过QA的其他电子受体的概率(ψ0)和t=0时用于电子传递的量子产额(φE0)在处理间均差异不显著(P>0.05),但三者与无氮和NH4Cl处理组相比均具有显著差异。(3)与NaNO2、NaNO3、CO(NH2)2处理组相比,无氮环境使得盐藻相对可变荧光(Vj)显著升高,盐藻光合电子从QA-到QB的传递受阻,导致QA-大量积累;而NH4Cl做氮源时,杜氏盐藻叶绿素荧光动力学曲线的K相出现,使得其PSⅡ放氧复合体(OEC)受损。研究认为,杜氏盐藻在NaNO3、CO(NH2)2、NaNO2作为唯一氮源时均可良好生长,并在CO(NH2)2环境中生长得更快;缺氮胁迫使得盐藻生长受到显著抑制,PSⅡ反应活性中心的数量降低,电子传递受阻;而NH4Cl对杜氏盐藻的毒性效应使得其PSⅡ放氧复合体(OEC)受损,藻体在短期内开始死亡。

    Abstract:

    In order to investigate the response of growth and PSⅡ of Dunaliella salina to different nitrogen sources, we performed experiments by using NaNO3, CO(NH2)2, NaNO2, and NH4Cl as nitrogen sources and N control was set in this study. Our results showed that: (1) D. salina grew faster under CO(NH2)2 with a maximum growth rate[μmax, (0.482±0.032)/d] per day than under other nitrogen sources. (2) Results of chlorophyll fluorescence indicated no significantly different in the initial slope of the RLC before the onset of saturation (α), the maximum potential ETR (rETRmax), the maximum photochemistry efficiency (Fv/Fm), the maximum quantum yield of primary photochemistry (φP0), quantum yield for electron transport (φE0), probability(t=0) that a trapped exit on moves an electron into the electron transport chain beyond QA- (ψ0), absorption flux per RC (ABS/RC) and trapped energy flux per RC(t=0) (TR0/RC) among NaNO3, CO(NH2)2, and NaNO2 groups (P>0.05), while there is significantly different in these parameters when compared with NH4Cl and N controls (P<0.05). (3) Compared with the NaNO2, NaNO3, and CO(NH2)2 groups, the relative variable fluorescence (Vj) of D. salina increased significantly under nitrogen free treatment (P<0.05), which suggesting restriction of photosynthetic electron transport of D. salina from QA- to QB and accumulation of QA-. However, we can infer destroy of the oxygen evolving complex (OEC) of D. salina by the NH4Cl from the appearance of K phase under NH4Cl. As a whole, these results showed that D. salina grew much faster under CO(NH2)2 than that under NaNO3 and NaNO2, whereas the growth of D. salina was inhibited significantly by nitrogen deficiency, and the number of reactive centers in PS II was decreased, and the electron transport was blocked. However, toxicity effect of NH4Cl on D. salina caused its death in a short time and the OEC of D. salina was damaged.

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杨宋琪,王丽娟,谢 婷,等.氮源对杜氏盐藻生长及光合系统Ⅱ的影响[J].西北植物学报,2017,37(7):1397-1403

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  • 在线发布日期: 2017-08-09
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