中间锦鸡儿CiABR17基因的克隆及表达分析
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国家自然科学基金(31360169);


Clone and Expression Analysis of CiABR17 Gene from Caragana intermedia
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    摘要:

    该研究采用blastn比对方法,从中间锦鸡儿干旱胁迫抑制消减杂交文库筛选出ABA响应蛋白17基因(ABR17)的EST序列。RACE扩增得到cDNA序列594 bp,其中ORF为471 bp,编码157个氨基酸,预测蛋白质分子量为16.44 kD,等电点为4.93,平均疏水指数为-0.126,是一种亲水蛋白。实时荧光定量PCR检测发现,中间锦鸡儿CiABR17基因在脱水、NaCl和热胁迫下均有不同程度表达,说明CiABR17基因与中间锦鸡儿响应逆境胁迫有关;过表达CiABR17拟南芥株系的鲜重、干重和莲座直径均极显著高于野生型株系。

    Abstract:

    In this study, we selected the EST sequence of CiABR17 from the suppression subtractive hybridization (SSH) library of Caragana intermedia under drought stress via blastn. Through RACE, we obtained the 594 bp cDNA fragment of ABR17. The open reading frame is 471 bp, which encodes a polypeptide of 157 amino acid residues, with a calculated molecular weight of 16.44 kDa and a pI of 4.93. The average hydrophobic index of ABR17 is -0.126, which is a hydrophilic protein. The quantitative realtime PCR analysis indicates that the transcript level of CiABR17 was induced differently by dehydration, NaCl and heat treatments, which showed that CiABR17 gene was related to stress response of C. intermedia. Significant difference between CiABR17 overexpression lines and wildtype in fresh weight, dry weight and rosette diameter was found.

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刘 坤,杨 杞,赵 娜,等.中间锦鸡儿CiABR17基因的克隆及表达分析[J].西北植物学报,2017,37(9):1703-1710

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  • 在线发布日期: 2017-10-23
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