Abstract:In this study, Glycine max ‘Zhonghuang 13’ cultivar was used as the experimental material. On the basis of screening suitable concentrations of NaCl and uniconazole (S3307), the relative physiological indexes and anatomical structures of roots, stems and leaves were compared by foliar uniconazole spraying in order to understand whether spraying fronds with S3307 can advance soybeans ability to cope with salt stress. The results indicated that: (1) TBARS contents in root and leaf were increased, and total chlorophyll and carotenoid contents were decreased significantly under salt stress. The anatomical structure of leaves showed that the leaf thickness was thinner with spongy and palisade tissue cells arranging in disorder under salt stress than control plants. For roots, salt stress caused that the proportion of cortex / root ratio became larger, but stele / root was smaller than controls. Additionally, the outer epidermis cells sagged inwards, and xylem vessel diameter decreased as compared to control plants, in partly containing the tylosis. The changes in stems were similar to those in roots. (2) Under salt stress plus leaf spraying of 80 mg/L S3307, TBARS contents, total chlorophyll and carotenoid contents were restored, and anatomical structures of roots, stems and leaves were between the control group and the salt treatment group. All these data indicated that spraying fronds with S3307 can advance salt tolerance of soybean, suggesting that it may be a usable approach to enhance salt tolerance of cultivated soybean in practice.