Abstract:In this research, we use GUS histochemical staining method to analyze the expression pattern of GUS gene of ProUPLs∶GUS transgenic plant, which initiate the GUS report gene by the promoter of HECT E3s genes (UPLs). The results of these six HECT E3s genes at different development stages and tissues show different expression patterns, but all of them have high activity at the early stage and senescence stage of leaf development. Interestingly, we find that the leaf shape of upl3 mutant show significant difference and both upl3 and upl4 mutant delay senescence and have different expression levels of senescence-associated genes compared to wild type. Thus, we speculate that both UPL3 and UPL4 are likely to regulate the leaf development and the progress of plant senescence.