Abstract:In order to further verify the function of cotton GhVHAA gene, we constructed the GhVHAA gene into the prokaryotic expression vector pET28a and induced by IPTG in E. coli BL21 (DE3) for resistance analysis. The results indicated that: (1) semiquantitative RTPCR analysis showed that the expression level of GhVHAA gene in cotton seedlings was induced by dehydration and salt stress. (2) A 1 872 bp coding region was ligated into the prokaryotic expression vector pET28a. The prokaryotic expression vector pET28aGhVHAA was successfully constructed. The SDSPAGE electrophoresis results show that there was a specific protein band at about 70 kD, which was identical with the expected molecular weight of the recombinant protein. (3) The resistance of recombinant bacterium BL21 (pET28aGhVHAA) to PEG6000 (20%) and NaCl (0.5 mol/L) was significantly higher than that of the control strain BL21 (pET28a), and indicating that the expression of GhVHAA gene in E.coli can enhance the resistance of the strain. The results provided a theoretical basis for the application of GhVHAA gene in plant stressresistant genetic engineering.