白番红花CrCBF1基因的克隆及功能分析
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

科技部重点研发计划(SQ2016YFSF030011);


Cloning and Function Identification of CrCBF1 Gene from Crocus alatavicus
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    该研究选取新疆地区耐寒植物白番红花为研究材料,采用RTPCR方法,从白番红花中克隆得到白番红花CBF1的全长cDNA序列,命名为CrCBF1(GenBank登录号MF681787)。结果表明,CrCBF1基因完整开放阅读框ORF长642 bp,编码213个氨基酸,分子量为23.8 kD,理论等电点为4.99,具有CBF家族典型的AP2保守结构域;亚细胞定位分析显示,CrCBF1基因编码的蛋白定位于细胞核;酵母自激活分析显示,CrCBF1转录因子具有转录激活活性;酵母功能验证分析显示,过表达CrCBF1基因可以明显提高酵母的抗寒性。

    Abstract:

    A CBF1like gene was isolated from Crocus alatavicus Regel et Sem, a perennial ephemeral plant distributed in high mountain of Xinjiang, China, by RTPCR method using a cDNA template. The gene is named as CrCBF1. Sequence analysis indicates that the open reading frame of CrCBF1(GenBank accession MF681 787) gene has 642 bp length, which encoding 213 amino acids with the molecular mass of 23.8 kD and the pI value of 4.99, the CrCBF1 protein encompasses conserved motif of AP2; The result of subcellular localization indicated that CrCBF1 protein was located in the nuclear, the transcriptional activation activity analysis in yeast indicated that CrCBF1 protein harbors the transcriptional activation domain and possess selfactivation ability; The result of function identification in yeast indicated that overexpression of CrCBF1 gene enhance the ability of coldresistance of recombinant yeast.

    参考文献
    相似文献
    引证文献
引用本文

张克闯,杨红兰,李小双,等.白番红花CrCBF1基因的克隆及功能分析[J].西北植物学报,2018,38(2):204-210

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-03-23
  • 出版日期:

微信公众号二维码

手机版网站二维码