Using ANOVA, correlation analysis and cluster analysis, we investigated six active components in leaves of 105 Eucommia ulmoides germplasm resources, discussed the genetic variation to provide theoretical basis for breeding and utilization of leafused E. ulmoides. The results showed that: (1) the variation of six active component contents in leaves were different among different E. ulmoides germplasm resources. The largest coefficient of variation appeared in isoquercitrin (34.42%) and the content ranged from 1.16 to 6.92 mg·g^-1, while the lowest coefficient of variation appeared in total flavonoids(19.35%) and the content ranged from 6.70 to 22.53 mg·g^-1. The diversity index of six active components were all above 2.0. The contents of six active components showed extremely significant difference (P＜0.01) among different germplasm resources. (2) There were significant differences in the contents of chlorogenic acid, geniposidic acid, asperuloside, isoquercitrin and total flavonoids among different origins. (3) Correlation analysis showed that the contents of aucubin, geniposidic acid and asperuloside were extremely significantly related to each other. The content of chlorogenic acid was extremely significant and positive correlated with aucubin, asperuloside, isoquercitrin and total flavonoids. No significant correlation was found between total flavonoids and other components except for isoquercitrin and chlorogenic acid. (4) E. ulmoides germplasm resources were divided into four groups according to the content difference of the six components, in which the group Ⅳ including 13 materials had higher contents of active components than that of other groups, which could provide basic material for breeding of E. ulmoides resources. Great variation and rich diversity of six active component contents were found in different E. ulmoides germplasm resources.