Dehydration time and thawing temperature were explored to test the possibility of encapsulationvitrification in the cryopreservation of bryophytes collected from moist habitats. The results showed that the regeneration rate of S. ovatum can reach 42.41% when the materials were precultured at 4 ℃ for 3 days, loaded with 60% PVS2 for 30 minutes and dehydrated with PVS2 for 60 minutes at 0 ℃, then stored in liquid nitrogen for 24 hours and thawed at 40 ℃ for 2 minutes. There are no significant differences of morphological indicators between the pants before and after cryopreserved. The results demonstrated the feasibility of cryopreservation of bryophytes in humid environment by encapsulationvitrification.