Using the main rose cultivars in Yunnan Province， ‘Carola’(red)，‘Movie Star’ (pink) and ‘Tineke’ (white) as the experimental materials, we researched the effects of drought stress on the petals relative moisture content, soluble protein content, membrane peroxidation and antioxidant enzyme activities. Our results showed that: （1）relative moisture content and soluble protein content of the pedals decreased during the drought stress, while the malondialdehyde (MDA) content kept increasing and reached the peak at the fifth day and Tinekes MDA content was the highest. （2）The activities of the petals superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) and glutathione reductase (GR), ascorbic acid peroxidase (APX) declined after rising. CAT, POD, APX and GR reached the maximum on the third day, which illustrated the petal cells had the highest abilities of removing reactive oxygen species(ROS). Then the pedals antioxidant capacity decreased sharply and the pedals began to senescence and die. The APX activities of three rose varieties did not decline significantly in the later period of drought stress, which indicated that APX played a more important role in drought resistance in the later treatment. The SOD activity of Corolla had the largest increase under the drought stress, and its average activity was also the highest among the three varieties. The POD activity of Corolla reached the maximum at the third day, which was 9.2 times of that of Tineke, indicating that the Corollas droughttolerant was stronger. （3）According to the average value of membership function, the drought resistance ability of rose is sorted, following as: Carola> Movie Star>Tineke. In conclusion, our results suggest that the three cut rose varieties enhance their droughttolerant ability by increasing the activities of antioxidant enzymes and the degree of peroxidation of petal membranes is aggravated by drought stress. Different varieties show different drought resistance, especially Carola displays higher droughttolerant ability.