欧洲卫矛秋冬转色期叶色变化的生理机制
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国家林业局“948”项目(2013430)


Physiological Characters of Euonymus europaea Leaves during the ColorChanging Period in Autumn and Winter
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    摘要:

    以3个欧洲卫矛(Euonymus europaea)品种‘矮生’(Euonymus europaea ‘Pumilis’)、‘八仙花’(E. europaea ‘Aldenhamensis’)和‘白果’(E. europaea ‘Albus’)为试验材料,测定其秋冬转色期叶色参数及各项生理指标的变化,分析色素与叶色参数、可溶性糖、相关酶的关系,探讨欧洲卫矛转色期叶片的呈色机理,为后期园林应用提供依据。结果表明:(1)‘八仙花’、‘白果’叶片明度参数L*值、色素参数b*值(黄/蓝)呈先升后降的单峰曲线,‘矮生’的L*值、b*值呈先降后升的单峰曲线,3个品种色素参数a*值(红/绿)在转色期间均不断上升。(2)各品种叶片叶绿素含量随生育期整体呈下降的趋势,主要是叶绿素a减少,叶绿素b与类胡萝素下降较为平稳;花色素苷含量、花色素苷/叶绿素比值呈上升趋势,类胡萝素/叶绿素比值整体呈下降的趋势。(3)各品种叶片可溶性糖含量随生育期呈先升后降的单峰曲线,它们的PAL活性呈波动上升趋势,POD活性整体呈下降趋势,PPO活性呈缓慢上升趋势。(4)相关性分析显示,3个品种叶色参数a*值与花色素苷含量均呈极显著正相关关系,‘矮生’花色素苷含量与叶绿素、类胡萝素含量呈显著负相关关系,‘八仙花’、‘白果花’的花色素苷含量与叶绿素、类胡萝卜素含量呈不显著负相关关系。研究发现,影响欧洲卫矛秋冬转色期叶片变红的主要因素是叶绿素、花色素苷和可溶性糖相对含量及PAL、PPO活性,类胡萝卜素含量对叶片变红的贡献不大,POD活性对叶片变红无直接影响。

    Abstract:

    With three 3yearold cutting seedlings of Euonymus europaeaE. europaea ‘Pumilis’, E. europaea ‘Aldenhamensis’, E. europaea ‘Albus’)varieties as test materials, changes of leaf color parameters and various physiological indicators were measured during the colorchanging period in autumn and winter. The relationships between pigment and leaf color parameters, soluble sugar, and related enzymes during the color change were analyzed. The mechanism of leaf color development during the color transformation period of Euonymus europaea was discussed to provide a basis for the application of garden in the later period. The results showed: (1) during the color change, the value of L* and b* of ‘Aldenhamensis’ and ‘Albus’ presented a single peak curve that rose firstly and then fell. The value of L* and b* of ‘Pumilis’ presented a single peak curve that drops firstly and then rises. The value of a* of three varieties rose continuously. (2) Chlorophyll showed a decreasing trend as a whole, mainly due to a decrease in chlorophyll a. Chlorophyll b and carotenoids decreased more steadily; Anthocyanin content and anthocyanin/chlorophyll ratio were on the rise. Carotenoid/chlorophyll ratio showed a decreasing trend as a whole. (3) Soluble sugar mass fraction showed a single peak curve that rose firstly and then fell; The activity of PAL in three varieties showed a rising trend; POD activity showed a downward trend; PPO activity is slowly rising. (4) The correlation analysis showed: The leaf color parameters a* value and anthocyanin content of three varieties were both presented extremely significantly positive correlation. Anthocyanin was significantly negative correlation with chlorophyll and carotenoids in ‘Pumilis’. Anthocyanin was no significantly negative correlation with chlorophyll and carotenoids in ‘Aldenhamensis’ and ‘Albus’. All in all, the main factors that affect the changes of leaves during the colorchanging period in autumn and winter in Euonymus europaea were the contents of chlorophyll, anthocyanin, soluble sugar and the activities of PAL and PPO. The content of carotenoid had minor effects while the POD activity had no direct effects.

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卓启苗,丁彦芬,余 慧,等.欧洲卫矛秋冬转色期叶色变化的生理机制[J].西北植物学报,2018,38(6):1072-1079

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  • 在线发布日期: 2018-07-11
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