7个烤烟产量相关性状的QTL定位分析
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中国烟草总公司项目[110201601027(JY01),110201801029(JY06)];


Mapping of Quantitative Trait Loci Underlying Seven Yieldrelated Traits in Fluecured Tobacco (Nicotiana tabacum L.)
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    摘要:

    以烤烟Y3和K326为亲本,采用单粒传法(SSD)获得一个包含262个F6株系的重组自交系群体(RILs)。基于该群体构建了一张含有24个连锁群、626个SSR标记,总长为1 120.45 cM的遗传图谱。通过两年一点3次重复的随机区组田间试验,测定了株高、节距、叶数、茎围、茎叶角度、腰叶长和腰叶宽7个与产量相关农艺性状,采用混合线性模型的严格复合区间作图法(rMQM)在烟草全基因组范围内进行QTL扫描分析。结果显示:(1)烤烟7个目标性状在RILs群体内各株系间存在较大范围的连续变异,具有显著的双向超亲分离,且各性状的平均值很接近中亲值; 7个农艺性状的平均广义遗传率为73.33%,其中株高和节距的广义遗传率在80%以上,而茎围和茎叶角度的广义遗传率则低于60%,表明7个与烤烟产量相关的农艺性状是既受微效多基因控制又受环境条件影响的数量性状。(2)共检测到30个QTLs分布在9条连锁群上,其中与株高、节距、叶数、腰叶长和腰叶宽相关的5个主效QTLs在连续两年中均可检测到,且具有较大的效应值,可解释大于10%的表型变异。(3)7个目标性状之间存在一定程度的相关性,与之对应在基因组中也存在一些较小区域,每个区域包含2个或2个以上紧密连锁的不同性状的QTLs。烟草产量相关农艺性状的QTL分析和主效QTLs的获得,为进一步利用分子标记辅助选择培育烟草高产新品种奠定了理论基础。

    Abstract:

    A population of 262 recombinant inbred lines (RILs) from a fluecured tobacco cross between Y3 and K326 by single seed descent (SSD) method was used to construct a genetic linkage map. 626 SSR loci were evenly mapped into 24 linkage groups (LGs) which spanned a total length of 1 120.45 cM. With a randomized complete block design, one location field tests with three replicates in two years were conducted to characterize seven agronomic traits related to leaf yield, including plant height (PH), internode length (IL), leaf number (LN), stem girth (SG), angle between leaf and stem (ALS), length of waist leaf (LWL) and width of waist leaf (WWL). Based on the data of field trials and rMQM method, we mapped and analyzed the QTLs for seven target characters. The results showed:(1)the seven target traits of fluecured tobacco had a wide range of continuous variation in the RILs population, with significant bidirectional superparental segregation, and the average value of each trait was very close to the midparent value. The average broad heritability of 7 agronomic traits was 73.33%, of which the generalized heritability of PH and IL was above 80%, while the SG and ALS was less than 60%, indicating that 7 agronomic traits related to fluecured tobacco yield were quantitative traits controlled by both microeffects and environmental conditions.(2)A total of 30 QTLs were detected on 9 linkage groups, while five major QTLs with relatively large effects, explaining for more than 10% of the phenotypic variation in the RILs population, related to PH, IL, LN, LWL, and WWL were detected by both two years. (3)The seven traits were largely correlated with each other. Consistent with this, some small regions harboring two or more closely linked QTLs of different traits were found in the genome. The results provide an important theoretical basis for developing high yield cultivars in tobacco through markerassisted selection (MAS).

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童治军,焦芳婵,陈学军,等.7个烤烟产量相关性状的QTL定位分析[J].西北植物学报,2018,38(7):1235-1243

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  • 在线发布日期: 2018-08-17
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