In this experiment, three different species of black currant ‘Adelinia’, ‘Brodtrop’ and ‘Heifeng’ were used as materials to study the growth of black currants leaves. The ascorbic acid (AsA) content, synthesis and metabolismrelated enzyme activities, and their relationship with each other in leaves of eight different development stages (leaf to senescence) of black currants were studied. The relationship between AsA content and enzymes related to metabolic synthesis during the process of leaf growth and natural aging was clarified, which laid the foundation for finding the best leaf picking stage, regulation of AsA content, and delaying the process of leaf senescence. The results showed: (1) AsA content and AsArelated metabolite levels in different varieties of black currant leaves have obvious diversity. During the leaf growth and development, the changes of total ascorbic acid (TAsA) and AsA contents in leaves were consistent among the three cultivars, and all of them reached the highest level in the mature leaves, and the AsA content in leaves of ‘Adelinia’ was the highest (98.33 μmol/g) at maturity, then declined rapidly with leaf senescence, and decreased to 34.57 μmol/g in the late senescence. (2) In the process of leaf growth and development, the change of Lgalactose1, 4lactone dehydrogenase (GalLDH) activity in the Lgalactose pathway was consistent with the change trend of AsA content. In the regenerative metabolism of AsAGSH, the activities of dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) are the highest in the leaf spreading stage, and then follow leaf senescence to the lowest. This is similar to the change trend of AsA content, but the change in the activity of ascorbate peroxidase (APX) is exactly the opposite. The content of H₂O₂ in leaves gradually increased with the senescence of the leaves and reached the highest level during the aging period. (3) GalLDH showed highly significant positive correlation with TAsA, AsA, oxidized ascorbic acid (DHA), DHAR, and MDHAR. The higher the GalLDH activity in the leaves, the higher the content of AsA in the leaves; DHAR is also significantly positively correlated with TAsA, AsA and DHA. Studies indicated that AsA content in black currant leaves reached its highest level in mature leaves, and there was a significant difference between the varieties; GalLDH, DHAR may be the key enzymes in AsA anabolism of black currant leaves, which can be enhanced by increasing GalLDH and AsAGSH. Circulatory systemrelated enzyme activities increase AsA content in black currant leaves, thereby delaying senescence.