Abstract:The fulllength cDNA sequence of the carotenoid cleavage dioxygenases CsCCD1 and CsCCD4 (NCBI accession number:MH119136 and MH119137) were cloned, respectively, by homology cloning techniques from the leaves of tea cultivar ‘Tieguanyin’. The sequence analysis showed that the length of CsCCD1 was 1 766 bp, which contained a 1 641 bp ORF encoding 545 amino acids. Prediction results showed that it did not contain the transmembrane structure and signal peptides, and had high probability to locate on the cytoplasm. Additionally, the length of CsCCD4 was 1 942 bp, which contained a 1 842 bp ORF encoding 612 amino acids. Prediction results showed that it did not contain the transmembrane structure but had a Nterminal chloroplast targeting transit peptides. Phylogenetic tree analysis showed that both CsCCD1 and CsCCD4 could be clustered into the same category with RjCCD1 and RjCCD4 of Rhododendron japonicum, respectively. Tissuespecific expression pattern analysis showed that both CsCCD1 and CsCCD4 were highly expressed in leaf, stem and flower. During the greenmaking procedure of Oolong tea, expression of gene CsCCD1 and CsCCD4 downregulated from leaves to solar withering. Expression of gene CsCCD1 upregulated from first tumblings to second tumblings, downregulated rapidly after third tumblings. Expression of gene CsCCD4 upregulated after first tumblings, and then drops rapidly. Indicate that they might be related to aroma formation in greenmaking procedure of Oolong tea.