In this paper, the strawberry plant growth, physiology, yield and quality were analyzed and compared under the condition of intercropping with corn and continuous cropping, with a purpose of providing the theoretical basis for overcoming the continuous cropping obstacles in strawberry production. Results showed that: (1) the strawberry growth index of intercropping with corn was significantly higher than that of continuous copping, such as the plant height, the root length, the stem diameter, and the weight of strawberry plant. (2) Compared with continuous cropping, the tillage pattern of intercropping with corn could increase the protective enzyme activities of superoxide dismutase, ethanol dehydrogenase and peroxidase. In addition, intercropping with corn could increase soluble sugar content in roots and leaves of strawberry plant, respectively, and make the malondialdehyde content in a relative low level, simultaneously. It means that intercropping with corn could ensure the strawberry plant growth well. (3) Intercropping with corn could reduce the rate of strawberry plants by 28.8%, when they were transplanted at the beginning of autumn, and these transplanted strawberry could be quickly recovered in the field of intercropping with corn, which compared with continuous cropping. (4) The strawberry plant, which intercropping with corn, has the ability to significantly increase their fruit hardness, the single fruit weight, and the yield of single strawberry plant concurrently, but has no significant effect on the soluble content. We can summarize that strawberry continuous cropping obstacles could be overcome by intercropping with corn, and the strawberry plant vegetative growth and the resistance to stress were improved, and the fruit yield and quality were also increased under the intercropping with corn, respectively.