Abstract:In this study, based on the result of the genome survey sequencing of Brassica juncea, we cloned four WRKY71s genes by RTPCR technology, which named BjuWRKY7114. Bioinformatics analysis showed that BjuWRKY7114 contained 822, 840, 834 and 855 bp ORFs, encoding 273, 279, 277 and 284 amino acid residues, respectively. It is predicted that the molecular mass of BjuWRKY7114 proteins were 30.80, 27.11, 31.58, 32.18 kD, which isoelectric point were 7.75, 9.08, 8.35 and 7.76, respectively. Phylogenetic tree analysis showed that BjuWRKY7114 had the highest similarity with BrWRKY71 transcription factor. Subcellular localization prediction indicated that BjuWRKY71s were localized to the nucleus. BjuWRKY7114 genes were located on the chromosome A09, B04, A07 and scaffold144, respectively. Results of qRTPCR analysis showed that four BjuWRKY71s genes were responsive to high salt, abscisic acid(ABA) and low temperature stress. Under salt stress, the expression of BjuWRKY713 was increased continuously, while the expression of the other three genes was the highest at 6 h, and then decreased. Under the treatment with ABA, the expression of BjuWRKY71s gene was the highest at 6 h and then decreased gradually. After low temperature treatment, the expression level of the BjuWRKY71s was increased significantly. BjuWRKY714 was the most responsive to the abiotic stresses. These results could provide a foundation on further explore the function of BjuWRKY71s in B. juncea tolerance to abiotic stress.