芥菜型油菜WRKY71转录因子基因家族的鉴定及表达分析
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国家自然科学基金(31560404);


Identification and Expression Analysis of WRKY71 Transcription Factor Family Genes in Brassica juncea
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    摘要:

    WRKY转录因子是植物中最大的转录因子家族之一,在植物生长发育及逆境胁迫中起重要作用。该研究在芥菜型油菜基因组概览测序的基础上,采用RTPCR技术克隆了4个芥菜型油菜WRKY71s基因,分别命名为BjuWRKY7114。生物信息学分析表明,BjuWRKY7114基因开放阅读框(ORF)分别为822、840、834和855 bp,分别编码273、279、277和284个氨基酸,预测分子量和等电点分别为30.80、27.11、31.58、32.18 kD和7.75、9.08、8.35、7.76。系统进化树分析表明,BjuWRKY7114与白菜WRKY71转录因子相似性最高。亚细胞定位预测分析结果显示,BjuWRKY71s蛋白均定位于细胞核。这4个基因分别定位到芥菜型油菜的A09、B04、A07和scaffold144上。qRTPCR结果表明,4个BjuWRKY71s基因在高盐、脱落酸和低温胁迫下都有响应。在盐胁迫下, BjuWRKY713的表达量呈持续上升趋势,而其他3个基因在6 h时表达量达到最大,然后降低;在ABA处理下,BjuWRKY71s基因于6 h时表达量最大,随后逐步降低;低温处理后,BjuWRKY71s基因受低温诱导都显著上调表达。BjuWRKY714在该研究的3个胁迫条件下响应最为敏感。研究结果为进一步探讨该基因家族对芥菜型油菜逆境调控机制奠定了基础。

    Abstract:

    In this study, based on the result of the genome survey sequencing of Brassica juncea, we cloned four WRKY71s genes by RTPCR technology, which named BjuWRKY7114. Bioinformatics analysis showed that BjuWRKY7114 contained 822, 840, 834 and 855 bp ORFs, encoding 273, 279, 277 and 284 amino acid residues, respectively. It is predicted that the molecular mass of BjuWRKY7114 proteins were 30.80, 27.11, 31.58, 32.18 kD, which isoelectric point were 7.75, 9.08, 8.35 and 7.76, respectively. Phylogenetic tree analysis showed that BjuWRKY7114 had the highest similarity with BrWRKY71 transcription factor. Subcellular localization prediction indicated that BjuWRKY71s were localized to the nucleus. BjuWRKY7114 genes were located on the chromosome A09, B04, A07 and scaffold144, respectively. Results of qRTPCR analysis showed that four BjuWRKY71s genes were responsive to high salt, abscisic acid(ABA) and low temperature stress. Under salt stress, the expression of BjuWRKY713 was increased continuously, while the expression of the other three genes was the highest at 6 h, and then decreased. Under the treatment with ABA, the expression of BjuWRKY71s gene was the highest at 6 h and then decreased gradually. After low temperature treatment, the expression level of the BjuWRKY71s was increased significantly. BjuWRKY714 was the most responsive to the abiotic stresses. These results could provide a foundation on further explore the function of BjuWRKY71s in B. juncea tolerance to abiotic stress.

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袁玉辉,王舸泓, 董浩然,等.芥菜型油菜WRKY71转录因子基因家族的鉴定及表达分析[J].西北植物学报,2018,38(10):1801-1808

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  • 在线发布日期: 2018-11-23
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