PcLIPH8基因水稻的构建及其相关表型分析
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国家自然科学基金河南省联合基金(U1704106);


Generation and Related Phenotype Analysis of the Transgenic PcLIPH8 Rice
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    摘要:

    利用黄孢原毛平革菌木质素过氧化物酶基因(PcLIPH8),构建植物双元表达载体35S∷PcLIPH8,并遗传转化水稻野生型品种Kitaake,对转基因水稻进行分子鉴定、酶活及木质素含量测定、表型观察等分析。结果表明:(1) 成功构建了植物双元表达载体35S∷PcLIPH8,获得3个独立的转PcLIPH8水稻株系,但在苗期转基因水稻与野生型对照无明显表型差异。(2) 酶活及木质素含量测定结果表明,转基因水稻的木质素过氧化物酶活性增加3.06%~5.07%,而木质素含量显著低于野生型对照,苗期降低11.44%~14.97%,成熟期降低13.83%~20.05%。(3) 成熟期表型分析表明,转基因水稻较野生型对照的株高增加了28.37%~39.78%,穗谷粒数增多110%~120%,生物量增大18.61%~22.97%,而千粒重减小12.86%~13.34%,谷粒长度变短6.67%~7.15%。该研究结果为利用PcLIPH8基因降低木质素含量,提高生物产量,从而改善植物品质奠定了前期基础。

    Abstract:

    Microbial lignin peroxidase is considered one of the key enzymes for lignin degradation. The lignin peroxidase gene (PcLIPH8) from Phanerochaete chrysosporium was introduced into plant binary expression vector for wildtype rice (Kitaake) transformation. Transgenic rice lines were confirmed by molecular identification. Enzymatic activity, lignin content determination and phenotypic changes were analyzed. The results showed that: (1) 35S∷PcLIPH8 plant binary expression vector was successfully transformed and three independent homozygous transgenic lines of rice were obtained. At seedling stage, there is no significant difference between transgenic rice lines and wildtype control. (2) Enzymatic activity and lignin content determination showed that lignin peroxidase activity increased 3.06%-5.07% and the lignin content decreased 11.44%-14.97% at seedling stage and 13.83%-20.05% at mature stage in transgenic rice lines compared with the wildtype control. (3) Phenotypic analysis at harvest showed that the plant height increased 28.37%-39.78%, panicle grain number increased 110%-120%, and biomass increased 18.61%-22.97% compared with the wildtype control. However, the grain weight decreased 12.86%-13.34%, and the grain length decreased 6.67%-7.15%. The research laid a foundation for the further use of the PcLIPH8 gene to reduce lignin content and increase biological production for improving plant quality.

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李 珅,王 珂,林法明,等.转PcLIPH8基因水稻的构建及其相关表型分析[J].西北植物学报,2018,38(11):1968-1974

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  • 在线发布日期: 2018-12-20
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