An anthocyanin synthesis related gene named LrTTG1 (GenBank accession number MH633481) was cloned from Lycium ruthenicum using RTPCR and RACE methods. The sequence analysis showed that the length of LrTTG1 gene was 1 453 bp, which contained a 1 029 bp ORF encoding 342 amino acids with five WD40 repeats. Homology amino acid sequences comparison indicated that LrTTG1 had higher similarity with Solanum melongena TTG1 (83.73%). qRTPCR analysis revealed that LrTTG1 gene was expressed in stems, leaves, flowers, green fruits, purple fruits and black fruits, and the expression level (the highest) in green fruits was about 4 times that of black fruits (the lowest). LrTTG1 gene expression showed a trend on firstly falling and then rising with UV irradiation time. Anthocyanin content analysis showed that the content in black fruits was the highest (11.3 mg/g), which were about 9.4 times and 21.3 times of purple fruits (1.2 mg/g) and green fruits (0.53 mg/g), respectively. This study showed that the expression of LrTTG1 gene tended to decrease with the development of fruits, while the content of anthocyanin tended to increase, showing a negative correlation between them. It is speculated that LrTTG1 gene may play an important role in the anthocyanin synthesis of L. ruthenicum.