固氮菌株XD20对不同甘蔗品种的促生效应
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国家自然科学基金(31560352,31471449);


Effect of NitrogenFixing Strain XD20 on the Growth of Different Sugarcane Varieties
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    摘要:

    菌株XD20是从甘蔗根内分离获得的一株具有固氮活性的内生菌株。该试验对其进行16S rDNA鉴定和nifH基因扩增,并对其固氮、溶磷、产嗜铁素、产吲哚乙酸(IAA)等促生特性进行分析,然后接种于甘蔗,研究其对甘蔗的促生效应。结果表明:(1)16S rDNA序列的分析鉴定显示,该菌株属于成团泛菌。(2)nifH基因通用引物polyF/polyR扩增得到一条大小为360 bp左右的基因序列,该菌的固氮酶活性约为3 187.8 nmol·mL-1·h-1。(3)菌株XD20具有产生IAA的能力,IAA浓度可达36.71 μg·mL-1,同时具有溶解有机磷和产生嗜铁素的能力。(4)接种XD20到不同甘蔗品种B8、ROC22和GT40的组培苗,接菌处理不同程度地提高3个品种甘蔗的株高、生物量以及叶片的叶绿素含量、硝酸还原酶活性、谷氨酰胺合成酶活性以及硝态氮的含量,并对品种B8的促生作用效果最为显著。

    Abstract:

    Strain XD20 was an endophytic strain with nitrogen fixation activity isolated from sugarcane roots. In this experiment, 16S rDNA identification and nifH gene amplification were carried out, and its growth promoting characteristics, such as nitrogen fixation, phosphorus dissolving, ferritin production and indoleacetic acid (IAA) were analyzed. Meanwhile, the growth promoting effects on sugarcane after inoculating were also studied. The results showed that: (1) strain XD20 was identified as Pantoea agglomerans by 16S rDNA gene sequence. (2) About 360 bp was amplified by universal primer polyF/polyR of nifH gene, and the nitrogenase activity of the strain XD20 was 3 187.8 nmol·mL-1·h-1 by using acetylene reductase method. (3) Strain XD20 had an ability to produce IAA, which the concentration of IAA reached 36.71 μg·mL-1, and also can dissolve organophosphorus and produce ferritin. (4) Inoculating the strain XD20 to different varieties of sugarcane (B8, ROC22 and GT40), increased the plant height, plant biomass, chlorophyll content in leaves, nitrate reductase activity, glutamine synthase activity and nitrate nitrogen content of three sugarcane cultivars at varying degrees, and the promoting effect of B8 was the most significant.

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毛莲英,黄 婵,祝 开,等.固氮菌株XD20对不同甘蔗品种的促生效应[J].西北植物学报,2019,39(1):140-148

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  • 在线发布日期: 2019-02-28
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