Goldenlike (GLK) transcription factors play an important role in the growth and formation process of plant leaves and fruit chloroplast. In this study, Solanum lycopersicum (AC) transformed with SlGLK1 and SlGLK2 genes (ACCS SlGLK1, ACCS SlGLK2 and ACOE SlGLK2) was used as the research material, to detect the content of chlorophyll and the expression analysis of related transcription factor genes such as SlGLK1, SlGLK2, TKN2 (Transcription Factor of Knotted Like Gene), TKN4 (Transcription Factor of Knotted Like Gene) and APRR2 (Arabidopsis Pseudo Response Regulator 2Like) in different developmental stages of green fruits, BRIX and total sugar in mature transgenic fruits. The results showed that: (1) the leaf color in cosuppressed SlGLK1 gene plants became obviously lighter, and the fruit color of overexpressed SlGLK2 turned dark green, which corresponded to the detection of chlorophyll content in fruit. The chlorophyll content in the top of cosuppressed SlGLK1 fruit was the lowest in all materials, but the bottom of fruits were 5-6 times higher in overexpressed SlGLK2 than that of AC. (2) 0.3% of BRIX and 5.34 mg/g of total sugar has been increased in overexpressed SlGLK2 gene fruits, respectively. (3) The qPCR of SlGLK1 and SlGLK2 genes showed that the expression in the transgenic materials was significantly increased more than 5 times, even in the cosuppressed transgenic lines. TKN2 might be located upstream of the SlGLK2 gene and regulate SlGLK2 transcription factor. Therefore, SlGLKs transcription factor played an important role in improving the quality of tomato fruit by regulating the synthesis of chlorophyll in leaves and fruits, especially in enhancing the green of tomato leaves and fruit and increasing the BRIX and total sugar in mature fruits.