Abstract:Two tissues, the bark located at downside of shoot site of cuttings and leaf located at top of shoot, were sampled to compare the transcriptome profiles between upright and inverted cuttings of P. yunnanensis in vegetative growth stage (August). The results showed:(1) a different transcriptome profiling changing induced by cutting inversion from the two tissues. A total of 358 587 unigenes was obtained by transcriptome sequencing. Then, 6 512 DEGs were selected in leaf samples and 12 020 DEGs were selected in bark samples. (2) In total, 552 DEGs were identified by KEGG databases in leaf tissue and assigned to 122 metabolic pathways. The main significant enriched pathways were plantpathogen interaction, amino sugar and nucleotide sugar metabolism, pentose and glucuronate interconversions, galactose metabolism, biosynthesis of amino acids, and ascorbate and aldarate metabolism. The changing USPase encoded by 39 DEGs impacted on cell wall metabolism of leaf tissue. (3)In bark tissue, 1 623 DEGs identified by KEGG databases were mapped to 128 pathways, among which carbon metabolism, ribosome, biosynthesis of amino acids, and phenylpropanoid biosynthesis were main enriched pathways. (4)Furthermore, a large number of DEGs were found in ROS metabolic reactions, including enzymes in Calvin cycle related to ROS production system and POD and cysteine synthase (i.e. cysK and MET17) related to ROS removal system have large number of DEGs, indicated a change of ROS concentration balance induced by inversion and a activated defend system in bark tissue of cuttings.