滇杨正/倒扦插苗叶片和树皮组织的转录组比较分析
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国家自然科学基金(31860219,31360184);


Comparative Transcriptome Analysis of Bark and Leaf Tissues between Upright and Inverted Cuttings of Populus yunnanensis
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    摘要:

    该研究以滇杨正、倒扦插苗为对象,于速生期(8月)收集插穗主枝萌发处下侧树皮组织和主枝的叶片组织进行转录组比较分析。结果显示:(1)正、倒扦插滇杨苗于2种组织中的转录调控变化存在差异;测序得到358 587条Unigenes,并于叶片组织和树皮组织中分别筛选得到6 512和12 020条DEGs。(2)KEGG在叶片组织中成功注释了552个DEGs,被分配至122个通路中;显著富集通路主要为植物病原菌互作、氨基糖和核苷酸糖代谢、戊糖和葡糖醛酸互换、半乳糖代谢、氨基酸生物合成、抗坏血酸和新陈代谢,其中39个DEGs编码了USPase,影响叶片正常的细胞壁代谢。(3)树皮组织中,分布于128个通路中的1 623个DEGs被KEGG成功注释,主要的富集通路为碳代谢、核糖体、氨基酸生物合成、苯丙素生物合成。(4)大量DEGs富集到与ROS代谢相关的作用酶中,包括ROS产生机制相关的卡尔文循环相关酶、ROS清除机制相关的氧化还原酶(POD)和半胱氨酸合成酶(如cysK和MET17)等。研究认为,上述关键酶类的基因变化,表明倒插扰乱了树皮组织的ROS浓度平衡,激发了倒插苗的生理保护机制。

    Abstract:

    Two tissues, the bark located at downside of shoot site of cuttings and leaf located at top of shoot, were sampled to compare the transcriptome profiles between upright and inverted cuttings of P. yunnanensis in vegetative growth stage (August). The results showed:(1) a different transcriptome profiling changing induced by cutting inversion from the two tissues. A total of 358 587 unigenes was obtained by transcriptome sequencing. Then, 6 512 DEGs were selected in leaf samples and 12 020 DEGs were selected in bark samples. (2) In total, 552 DEGs were identified by KEGG databases in leaf tissue and assigned to 122 metabolic pathways. The main significant enriched pathways were plantpathogen interaction, amino sugar and nucleotide sugar metabolism, pentose and glucuronate interconversions, galactose metabolism, biosynthesis of amino acids, and ascorbate and aldarate metabolism. The changing USPase encoded by 39 DEGs impacted on cell wall metabolism of leaf tissue. (3)In bark tissue, 1 623 DEGs identified by KEGG databases were mapped to 128 pathways, among which carbon metabolism, ribosome, biosynthesis of amino acids, and phenylpropanoid biosynthesis were main enriched pathways. (4)Furthermore, a large number of DEGs were found in ROS metabolic reactions, including enzymes in Calvin cycle related to ROS production system and POD and cysteine synthase (i.e. cysK and MET17) related to ROS removal system have large number of DEGs, indicated a change of ROS concentration balance induced by inversion and a activated defend system in bark tissue of cuttings.

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邹辛联,周安佩,纵 丹,等.滇杨正/倒扦插苗叶片和树皮组织的转录组比较分析[J].西北植物学报,2019,39(2):240-249

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  • 在线发布日期: 2019-03-22
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