In this study, three photobiont (A, B, C) isolated and cultured in the lichens were used as research materials. The light microscopy, BCO, dithizone, xylenol orange and Potassium iodiderhodamine Bspectrophotometric method were used to determine the cell viability of photobiont, different heavy meatal contents in culture medium and photobiont. The absorption characteristics and tolerance of three photobionts to heavy metal Cu²⁺, Zn²⁺, Pb²⁺ and Cd²⁺ were analyzed to determine the relationship between photobiont and heavy metal stress, and to provide a theoretical basis for bioremediation of heavy metal polluted water. The results showed that: (1) under the different concentrations of heavy metal stress, the tolerance of three photobionts to four heavy metals：Zn²⁺ >Pb²⁺ >Cu²⁺ >Cd²⁺ and heavy metal toxicity of different photobionts: Cu²⁺(B> A >C)，Zn²⁺(B > A >C)，Pb²⁺(B > A >C), Cd²⁺(B > C > A).(2)All of the photobionts adsorbability of Zn²⁺ and Pb²⁺ was higher than that of Cu²⁺ and Cd²⁺, and the absorption of algal B to different heavy metals was higher than that of A and C. (3) All of the photobionts was positive correlated with cell absorption and tolerant concentration and different culture time points (P<0.01), and the tolerance of the two algal species to heavy metals was positively correlated with their absorption. The study found that the photobionts have a better tolerance and absorption to heavy metals than some freegrowing algae, will be a good material for remediation of heavy metal contaminated biosorbents, and the results of this study provide a theoretical basis for the practical application of photobiont in the bioremediation of water and metal pollution.