Abstract:FT (Flowering Locus T) and its homologous gene were considered to be important genes which related to plants blossom and flower development. To better study the function of the FT homologous gene and flowering mechanism of Oncidium ‘Jinhui’, we isolated the FT homologous gene named OnFT and its GenBank accession number was MK967676 on the basis of transcriptome sequencing results in this study. OnFT contains a 537 bp open reading frame which encoding 178 amino acids. Bioinformatic analysis showed that the molecular weight of OnFT protein was 19.99 kD and this protein might be a unstable hydrophilic protein. Besides, the OnFT protein belonged to the PEBP family. The phylogenetic analysis showed that Oncidium was closely related to Phalaenopsis equestris from Orchidaceae. qRTPCR results indicated that the relative expression of OnFT gene were different in four tissues, with the highest expression was in flowers and almost no expressed in root. The expression level of OnFT gene in young flower buds was low and increased gradually during flower maturation. The expression of OnFT gene in leaves and pseudobulbs increased first and then decreased with the growth of maturity, highly expressed in leaves of middle seedling stage and pseudobulbs of budding stage.