Abstract:CinnamoylCoA reductase (CCR) is the key enzyme and plays an important role in the biosynthesis of lignin monomer. In this study, one CCR gene—HtCCR1 (GenBank accession number MN205540) was cloned from the Helianthus tuberosus variety ‘Lang Yu 8’. The open reading frame (ORF) of HtCCR1 was 975 bp, which encoded 324 amino acids. The HtCCR1 protein harbored the FR_SDR_e conserved domain. Phylogenetic analysis showed that HtCCR1 was closely related to the Helianthus annuus CCR protein (XP_021989763.1). The gene expression pattern and responses to salt and drought stresses of HtCCR1 were detected through qRTPCR. The result showed that HtCCR1 was continuously expressed in root, stem, leaf and tuber, and the expression in stem and leaf was the strongest; the expression of HtCCR1 in treatment group was significantly higher than that in control group at 6, 12 and 24 h after salt treatment; the transcription of HtCCR1 was significantly upregulated after 6 and 12 h of drought treatment. The prokaryotic expression vector of HtCCR1 (pET28aHtCCR1) was constructed and transformed into Escherichia coli BL21 (DE3), and after IPTG induction, the recombinant protein of the expected size was expressed. These results provided a basis for the further study on function of HtCCR1 and the regulation of lignin biosynthesis in Helianthus tuberosus using genetic engineering.