Abstract:In this study, the CsGME1 gene was cloned from cDNA of Camellia sinensis cv. ‘Longjing43’ based on the transcriptome and genome database of tea plant. Its protein characteristics and gene expression patterns were analyzed. In addition, the expression profiles of the CsGME1 under abiotic stress treatments in tea plant were detected by quantitative real time PCR. The main results listed below: (1) sequence analysis showed that the length of CsGME1 open reading frame (ORF) of tea plant was 1131 bp, encoding 376 amino acids. Multiple alignments of CsGME1 with related species showed that the identity of them was 94.25%, and all contain NAD binding domain. (2) Analysis of phylogenetic tree indicated that the CsGME1 was closely related to SlGME1 from Solanum lycopersicum, but far from OsGME2 of Oryza sativa. (3) Protein analysis suggested that the CsGME1 is a hydrophilic protein with the theoretical relative molecular weight 42 046.84 Da and the theoretical isoelectric point 5.73. CsGME1 protein had 4 disordered regions with low degree of disorder. Secondary structure prediction showed that the CsGME1 protein consists of 39.25% αhelix, 13.26% extended strand, 5.84% βturn and 41.38% random coil. The threedimensional structures indicated that CsGME1 protein contains helix and random coil. (4) The results of quantitative real time PCR showed that the CsGME1 gene responded to four kinds of abiotic stress treatments: high temperature (38 ℃), low temperature (4 ℃), drought (20% PEG) and high salinity (200 mmol·m-1 NaCl). The expression profiles showed difference.