The TLP15 was cloned from the resistant variety of Chinese wild Vitis quinquangularis ‘Shang24’ and the susceptible variety of Vitis vinifera cv. ‘Red Globe’ by RTPCR, named VqTLP15 and VvTLP15 (GSVIVT01018769001), respectively. We studied the gene by bioinformatics analysis, subcellular localization analysis, transformed Arabidopsis thaliana to observe and analyze transgenic Arabidopsis resistance to different pathogens. We also analyzed the expression of related genes involving in the SA or JA/Eth signaling pathways and regulating stomatal movement by qRTPCR. The results showed: (1) the open reading frame (ORF) of the VqTLP15 was obtained. VqTLP15 showed 98.99% and 99.66% sequence homology to VvTLP15 cloned from V. vinifera cv. ‘PinotNoir’ and VvTLP15 cloned from V. vinifera cv. ‘Red Globe’ by amino acid sequence alignment, respectively. (2) Subcellular localization indicates that VqTLP15 was localized in the cytoplasm. (3) VqTLP15 transgenic A. thaliana (L₁, L₂, L₃) were successfully obtained. (4) Inoculation observation showed that the transgenic A. thaliana lines were inoculated with G. cichoracearum and found to have greater resistance than Col0 to powdery mildew at 7 days postinoculation, and the concentration of powdery mildew spores from infected leaves were significantly lower in the transgenic lines than that in Col0. The leaf necrotic damage (43%) induced by Botrytis cinerea was significantly greater than that of Col0 in transgenic lines (71%, 62% and 67% of lesion area > 40% in L₁, L₂ and L₃, respectively). After PstDC3000 inoculation, the disease symptoms were less apparent in the transgenic lines than in Col0. The reduction of leaf stomatal aperture were greater in the transgenic lines than in Col0, and the concentration of bacteria was lower in the transgenic lines than in Col0. (5) A histochemical staining assay showed: Callose deposition was more widely distributed, and the frequency of cell death and O₂^-· levels were higher in the transgenic lines than in Col0 after powdery mildew inoculation. The extent of cell death, and levels of H₂O₂ and O₂^-· were higher in the transgenic lines than in Col0 after B. cinerea inoculation. After PstDC3000 inoculation, the frequency of cell death and the degree of O₂^-· accumulation were both higher in the transgenic lines than in Col0. (6) qRTPCR results showed: after powdery mildew inoculation, the expression levels of both PR1 and ICS1 increased in the transgenic lines, PR1 expression peaked at 72 hpi, and ICS1 peaked at 120 hpi. And the expression levels of LOX3 gradually decreased to the lowest level at 120 hpi in the transgenic lines, but remained higher than in Col0. The expression levels of PR1, NPR1 and PDF1.2 all increased following B. cinerea inoculation, and peaked at 48 hpi, and the expression levels of LOX3 decreased, but remained higher than in Col0. After PstDC3000 inoculation, PR1, PDF1.2 and NHL10 expression were all more highly expressed in the transgenic lines than in Col0, but WRKY53 expression was lower in the transgenic lines than in Col0. The expression levels of COI1, FRK1, ATPPC2, FLS2 and OST1 were higher in L₁ than in Col0 following PstDC3000 inoculation. After treatment with flg22 or LPS, the expression of COI1 was lower in L₁ than in Col0, but the expression levels of ATPPC2, FLS2, OST1 were higher in L₁ than in Col0. This study showed that overexpression of the VqTLP15 reduced the sensitivity to powdery mildew and PstDC3000 and increased the sensitivity to B. cinerea. VqTLP15 may involve in the defense response of plants via mediating salicylic acid (SA) and jasmonic acid / ethylene (JA / Eth) signaling pathways and stomatal immune response, and may present a candidate for future grape molecular breeding for disease resistance.