叶面喷施亚精胺对高温胁迫下番茄叶绿素合成代谢的影响
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重庆市教委科学技术研究项目(KJ1710242);


Effects of Foliarspraying Spermidine on Chlorophyll Synthesis Metabolism of Tomato Seedlings under Heat Stress
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    摘要:

    以设施番茄栽培品种‘金棚朝冠’幼苗为试验材料,研究叶面喷施0.25 mmol·L-1亚精胺(Spd)对高温胁迫下(38 ℃/28 ℃,昼/夜)番茄幼苗生长及叶绿素合成过程中前体物质含量、关键酶活性和叶绿素含量的影响,探讨Spd缓解番茄高温胁迫伤害的生理机制。结果表明:(1)高温胁迫显著抑制了番茄幼苗的生长,叶面喷施Spd可有效缓解高温胁迫对番茄幼苗地上部生长的抑制作用,但对于地下部的生长无显著缓解作用。(2)高温胁迫下番茄叶片叶绿素合成前体物质δ氨基酮戊酸(ALA)和胆色素原(PBG)的含量显著提高,而尿卟啉原Ⅲ(UroⅢ)、原卟啉Ⅸ(ProtoⅨ)、镁原卟啉Ⅸ(MgprotoⅨ)和原叶绿素酸(Pchl)的含量显著降低,证明叶绿素前体由PBG向UroⅢ的转化受阻,导致叶绿素a(Chla)、总叶绿素(Chlt)含量和Chla/Chlb显著降低。(3)叶面喷施Spd能增强高温胁迫下胆色素原脱氨酶(PBGD)活性,有效缓解了高温胁迫对PBG到UroⅢ转化的阻碍作用,促进PBG之后叶绿素合成前体物质的合成,Chla含量和Chla/Chlb显著增加。研究发现,高温胁迫显著抑制番茄幼苗的生长,叶面喷施Spd可通过显著增强PBGD活性来缓解由PBG向UroⅢ的受阻程度,促进高温胁迫下番茄叶片的叶绿素前体合成,提高叶绿素含量和番茄植株的生长量,减轻高温胁迫对番茄幼苗生长的伤害。

    Abstract:

    With tomato cultivar ‘Jinpengchaoguan’ as the experimental material, foliarspraying 0.25 mmol·L-1 spermidine (Spd) were applied to the seedlings, which grows under high temperature condition (38 ℃/28 ℃, day/night). The effects of foliarspraying Spd on the growth, the contents of chlorophyll precursors, key enzyme activity and the content of chlorophyll of tomato seedlings were discussed. The results showed that: (1) foliarspraying Spd significantly alleviated the decrease of shoot weight of tomato seedlings under heat stress, but it had no significant effect on root weight. (2) The contents of chlorophyll precursors δaminolevulinic acid (ALA) and porphobilinogen (PBG) in tomato leaves increased significantly under heat stress, but the contents of uroporphyrinogen Ⅲ (UroⅢ), protoporphyrin Ⅸ (Proto Ⅸ), Mgproto Ⅸ (Mgproto Ⅸ) and protochlorophyll (Pchl) decreased significantly under heat stress. The results indicated that chlorophyll synthesis blocked site was from PBG to UroⅢ, which might cause the reduce of chlorophyll a (Chla), total chlorophyll (Chlt) and Chla/Chlb. (3) Foliarspraying Spd increased the activity of porphobilinogen deaminase (PBGD) of seedlings under heat stress, which effectively alleviated the hindrance of heat stress on the transformation from PBG to UroⅢ, and promoted the synthesis of chlorophyll precursors after PBG. In summary, this study showed that tomato growth and chlorophyll synthesis were significantly inhibited under heat stress. This inhibition could be relived through foliarspraying Spd, which could alleviate the transformation from PBG to UroⅢ. This process promoted the synthesis of chlorophyll precursors, resulting in increased amount of chlorophyll and less inhibited growth of tomato plants under heat stress.

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向丽霞,胡立盼,孟 森,等.叶面喷施亚精胺对高温胁迫下番茄叶绿素合成代谢的影响[J].西北植物学报,2020,40(5):846-851

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  • 在线发布日期: 2020-07-09
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