Abstract:In order to explore the function of wheat (Triticum aestivum L.) WRKY gene, we cloned two WRKY genes named TaWRKYⅢA37 and TaWRKYⅡcD2 from wheat variety ‘Kenong 199’ by homologous cloning, and their bioinformatics and expression under different stresses were analyzed. Bioinformatic analysis showed that TaWRKYⅢA37 and TaWRKYⅡcD2 genes contain two introns and three exons, encoding 206 and 138 amino acids, respectively. The encoded proteins are all hydrophilic unstable non secretory nucleoproteins. Phylogenetic analysis showed that TaWRKYⅢA37 protein was the closest to its two homologous copies, while TaWRKYⅡcD2 protein was the closest to that of Aegilops tauschii. The results of qRTPCR showed that TaWRKYⅢA37 and TaWRKYⅡcD2 genes were expressed in the roots, stems and leaves of wheat. The former had the highest expression level in the roots, while the latter had the highest expression level in the leaves, all of which were low expression in the stems. The expression of TaWRKYⅢA37 gene in seedling stage was upregulated by PEG, H2O2 and ABA stress, and down regulated within 4~8 hours after NaCl treatment, which was lower than that of the control; While the expression was up regulated by PEG, NaCl, H2O2 and ABA in the filling stage. At seedling stage, TaWRKYⅡcD2 gene was down regulated by NaCl, H2O2 and ABA stress, and up regulated by PEG treatment for 2 hours, which was higher than that of control; While the expression was down regulated by PEG and NaCl stress at the filling stage, and up regulated by H2O2 and ABA stress. The results of this study lay a theoretical foundation for further exploring the antistress function of TaWRKYⅢA37 and TaWRKYⅡcD2 genes.