The proline transporter protein (ProTs) family plays an important role in proline distribution and protective stress responses in plants. To understand the profile of ProTs in tea plant, we used the bioinformatics methods to identify the members of CsProTs from genome of tea plant, and analyzed their phylogenetic relationship, protein structure and gene expression patterns in the present study. The results showed that： (1) there were six CsProT genes in tea plant: CsProT1, CsProT2, CsProT3, CsProT4, CsProT5 and CsProT6; We found that the number of amino acids encoded by CsProTs genes in tea plant is ranged from 441 to 574, the IP is covered from 8.51 to 9.41 and the molecular weight is distributed from 48.5 to 63.0 kDa; Most of them are basic proteins, which containing a large number of αhelix, free curls, a small number of elongation chains and βcorner structures. (2) The subcellular localization analysis showed that CsProT1, CsProT2, CsProT4, CsProT5 and CsProT6 were located in cell membrane, but CsProT3 located in Golgi. (3) The prediction results from amino acid sequences showed that each of CsProTs contained 9-11 transmembrane domains. The conservative motif analysis showed that the ProTs had high conservation in tea plant and Arabidopsis, and the consistency of amino acid sequence among different members was as high as 40.14%. (4) CsProT1, CsProT2 and CsProT3 genes were highly expressed in all tissues of tea plant, but CsProT4, CsProT5 and CsProT6 genes were low; And except CsProT5 gene, CsProTs genes were all induced by NaCl, drought and cold stress in the leaves of tea plant. (5) The proteinprotein interaction analysis showed that CsProTs could interact with proline oxidase ERD5, proline biosynthesis rate limiting enzymes P5CS1, P5CS2, δpyrroline5carboxylic acid dehydrogenase ALDH12A1 and other proteins related to proline synthesis, transport, degradation and jointly regulate proline content in tea plant. In conclusion, there were six CsProTs in tea plant, which are widely involved in the regulation of proline homeostasis and responding to different abiotic stress.