Abstract:Metal tolerant protein (MTP) maintains metal homeostasis in plants by absorbing and expelling metals from the cytoplasmic sol. In this study, we used a variety of bioinformatic methods to identify and analyze the TaMTP genes in the wheat genome, and qRTPCR to analyze the expression of TaMTP genes under the stress of various heavy metals, so as to provide theoretical basis for indepth study of the regulatory mechanism and stress resistance of this family of genes on the growth and development of wheat. The results showed that: (1) TaMTPs all had cationic Efferent family domain, and most members had zinc transporter dimeric domain. Phylogenetic and cluster analysis showed that TaMTP proteins were divided into seven groups: G1, G5, G6, G7, G8, G9 and G12. Analysis of gene structure and motif revealed that most TaMTP genes had relatively conservative exonintron arrangement and conserved motif composition. (2) Gene expression profile analysis of RNASEQ data showed that different TaMTP genes had their own unique expression mechanism, among which TaMTP11Bb and TaMTP11D had higher expression levels under abiotic stress, TaMTP11A, TaMTP11Bb, TaMTP11D, TaMTP113Ab, TaMTP113B and TaMTP113D had higher expression levels under biological stress. (3) qRTPCR analysis showed that when wheat was subjected to heavy metal stress of zinc (Zn), copper (Cu), cobalt (Co), cadmium (Cd), manganese (Mn) and iron (Fe), the expression levels of TaMTP11A、TaMTP84A、TaMTP84D、TaMTP113Aa and TaMTP113B were increased. The results showed that each metal ion could induce the expression of these TaMTP genes in roots and leaves. However, the expressions of TaMTP11A and TaMTP84A under Fe3+ and Cu2+ stress were completely opposite, and the expression levels of the two genes in wheat leaves and root tissues were low under Fe3+ stress. It was speculated that TaMTPs might be involved in the tolerance or transport of corresponding trace elements, but different TaMTP had different transport functions for different metals.