Sucrose transporter (SUT) plays an important role in the transportation and distribution of sucrose from source to sink. In this study, based on the latest published genomic data of upland cotton, we carried out the complete genome identification of SUT gene family, and systematically analyzed their expression characteristics by bioinformatics and qRTPCR method in upland cotton. Our results showed that: (1) a total of eighteen GhSUT genes (GhSUT1-_GhSUT18) were identified, and were unevenly distributed on 11 chromosomes in upland cotton. (2) The sequence identity of GhSUT proteins was very high, and all of them had 11-12 membranespanning domains, and were located on the plasma membrane. (3) Phylogenetic analysis indicated that the GhSUT protein was mainly distributed in the dicotsspecific SUT1 subgroup， and SUT2 and SUT4 subgroup which contained both dicotyledon and monocotyledon plants. Among them, SUT1 subgroup had the most members, including 8 GhSUT genes. (4) GhSUT genes within the same subgroup demonstrated similar intronexon organization. However, the number of intron/exon varied greatly among different subgroups. (5) Transcriptome analysis showed that the expression level of GhSUT genes was different. GhSUT1 and GhSUT10 could not be detected in the tested tissues. The expression level of GhSUT5, GhSUT14, GhSUT7 and GhSUT16 was low in the tested tissues, and other GhSUT genes had high expression level in the tested tissues. In addition, GhSUT gene had tissuespecific expression pattern, in which GhSUT2 and GhSUT11 were mainly expressed in source and sink organs, GhSUT6 and GhSUT15 were mainly expressed in sink organs, while GhSUT9 and GhSUT18 were mainly highly expressed in fibers. (6) Further qRTPCR analysis showed that GhSUT2 was highly expressed in the source and sink organs. GhSUT6 was mainly expressed in the roots, petals, fibers and stems of sink organs, but very low in the source organs (leaves). GhSUT18 was highly expressed in the fibers and very low in other tissues. The results showed that the experimental results were consistent with those of transcriptome analysis. The results of this study provided important genetic information for further study on the functions of SUT family genes, and laid a theoretical basis for improving yield and quality in cotton.