杧果Whirly基因鉴定及其在病菌侵染过程中的表达分析
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国家重点研发专项(2019YFD1000504);


Identification of Mango Whirly Gene and Its Expression Analysis in the Pathogen Infection
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    摘要:

    Whirly 家族是一类植物特异的转录因子,无论在细胞核还是在细胞器内都有着广泛而复杂的生物学功能。该研究以杧果全基因组数据为基础,采用生物信息学的方法对杧果Whirly家族基因进行序列分析,并通过qRTPCR技术分析杧果胶孢炭疽菌(Colletotrichum gloeosporioides,Cg)和细菌性黑斑病菌(Xanthomonas campestris pv. mangiferaeindicae,Xcm)侵染过程中杧果Whirly家族基因的相对表达量。结果表明:(1)从杧果基因组中鉴定了3个Whirly基因家族成员,分别命名为MiWHY1、MiWHY2和MiWHY3。(2)杧果Whirly蛋白均为不稳定亲水碱性蛋白;系统发育树显示,杧果Whirly基因与木薯、毛果杨、番茄Whirly基因亲缘关系最近,杧果与木薯、毛果杨、番茄Whirly家族共有1个高度保守的基序Motif1;杧果Whirly家族均含有Whirly超家族保守域,主要结构元件为无规则卷曲和α螺旋;保守结构域的四聚体结构与马铃薯Whirly蛋白四聚体结构具有高度相似性。(3)qRTPCR结果显示, Cg侵染过程中,杧果Whirly基因的相对表达量均显著上调;Xcm侵染12 h时,MiWHY1和MiWHY3相对表达量显著上调,初步确定杧果Whirly基因表达响应胶孢炭疽菌和细菌性黑斑病菌的侵染。研究认为,杧果中有3个Whirly基因家族成员,病菌侵染过程中可激发其表达活性,为后续研究杧果Whirly基因家族成员的功能和机制奠定基础。

    Abstract:

    Whirly family is a kind of plantspecific transcription factors, which play myriad roles both in the nucleus and organelles. Based on the whole genome data of Mangifera indica(mango), using bioinformatics methods to analyze the sequence of mango Whirly family genes, we studied the relative expression level of Whirly gene family by qRTPCR during the infection of Colletotrichum gloeosporioides(Cg) and Xanthomonas campestris pv. mangiferaeindicae(Xcm). The results showed that: (1) three Whirly family members, named MiWHY1, MiWHY2 and MiWHY3, were identified from the genome of mango. (2) The proteins of Whirly in mango were unstable hydrophilic basic proteins. The phylogenetic tree shows that the Whirly genes of mango are closely related to the Whirly genes of Solanum lycopersicum, Populus trichocarpa and Manihot esculenta, and there was a highly conserved sequence Motif1 in mango and Solanum lycopersicum, Populus trichocarpa and Manihot esculenta Whirly family. The Whirly family of mango contains the Whirly superfamily conservative domain, the main structural components of which are random coil and αhelix. The tetramer structure of conservative domain is similar to the tetramer structure of potato Whirly proteins. (3) qRTPCR results showed that the relative expression of mango Whirly genes were significantly upregulated during Cg infection. The relative expression levels of MiWHY1 and MiWHY3 were significantly upregulated at 12 h during Xcm infection. It was preliminarily determined that the mango Whirly gene expression responded to the infection of Cg and Xcm. In conclusion, there are three Whirly gene family members in mangosteen, and their expression level is stimulated during the infection process of pathogenic bacteria, which lays the foundation for the followup study of the functions and mechanisms of the mango Whirly gene family members.

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孙 宇,刘志鑫,叶 子,等.杧果Whirly基因鉴定及其在病菌侵染过程中的表达分析[J].西北植物学报,2021,41(1):37-45

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  • 在线发布日期: 2021-02-26
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