漆树TvMYB1基因的鉴定及干旱响应潜力分析
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引用本文:李梦歌,黄晓华,李大培,何学高,谢冬冬,王武萍,杨桂燕.漆树TvMYB1基因的鉴定及干旱响应潜力分析[J].西北植物学报,2021,41(3):392~397
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李梦歌1,黄晓华1,李大培1,何学高2,谢冬冬1,王武萍3,杨桂燕1* (1 西北农林科技大学 林学院陕西杨陵 7121002 国家林业和草原局西北调查规划设计院西安 7100003 中华全国供销合作总社西安生漆涂料研究所西安 710000) 
基金项目:国家重点研发计划(2017YFD0600705)
中文摘要:该研究筛选漆树MYB转录因子家族成员,经同源比对、开放读码框(ORF)、序列基本特征等分析,选取其中的TvMYB1进行基本生物学信息及逆境表达分析。以一年生漆树(Toxicodendron vernicifluum)盆栽苗为材料,采用RT PCR技术克隆漆树TvMYB1基因的cDNA,以漆树茎和根部的cDNA为模板对漆树TvMYB1基因进行克隆,采用实时荧光定量(qRT PCR)分析经PEG6000胁迫后其在根部、茎、叶片中随处理时间的表达变化。结果表明:(1)生物信息学分析显示,TvMYB1基因ORF长900 bp,编码蛋白含299个氨基酸,分子质量为32.45 kD,理论等电点为9.51,其蛋白结构含有1个MYB不完全重复子结构域,属于1R MYB类,亚细胞定位在细胞核内。(2)进化树分析显示,TvMYB1基因编码蛋白与阿月浑子(Pistacia vera)、可可豆(Theobroma cacao)、柑橘(Citrus sinensis)的MYB蛋白具有较近的亲缘关系。(3)qRT PCR分析结果显示,经PEG6000处理后,TvMYB1在漆树不同组织中均可被诱导表达,在叶片中的表达量最高,而在根和茎中表达量相对较低。随处理时间延长,TvMYB1在根部和茎中的表达有递增的趋势,在叶片中则相反。研究认为,TvMYB1受渗透胁迫诱导,在漆树干旱响应中具有一定作用,是漆树逆境适应机制研究的重要候选基因。
中文关键词:漆树  MYB转录因子  干旱胁迫  qRT PCR
 
Identification of the TvMYB1 Gene of Toxicodendron vernicifluum and Analysis of Its Drought Response Potential
Abstract:In this study, we screened the members of the MYB transcription factor family of T. vernicifluum. After homology comparison, open reading frame (ORF), basic sequence characteristics, etc., TvMYB1 was selected for basic biological information and stress expression analysis. Toxicodendron vernicifluum potted seedlings were used as materials, RT PCR technology was used to clone the cDNA of the TvMYB1 gene of the lacquer tree, and the TvMYB1 gene of the lacquer tree was cloned using the cDNA of the stem and root of the lacquer tree as a template, and the real time fluorescence quantitative (qRT PCR) analysis was used. After PEG6000 stress, its expression changes in roots, stems and leaves with the change of treatment time. The results showed: (1) bioinformatics analysis showed that the ORF of the TvMYB1 gene is 900 bp long, the encoded protein contains 299 amino acids, the molecular mass is 32.45 kD, and the theoretical isoelectric point is 9.51. The protein structure contains 1 incomplete MYB repeat, the subdomain belongs to the 1R MYB class, and is subcellularly located in the nucleus. (2) Phylogenetic tree analysis showed that the protein encoded by the TvMYB1 gene is closely related to the MYB protein of pistachio (Pistacia vera), cocoa (Theobroma cacao), and citrus (Citrus sinensis). (3) The results of real time fluorescent quantitative PCR (qRT PCR) showed that after PEG6000 treatment, TvMYB1 can be induced to express in different tissues of T. vernicifluum, with the highest expression in leaves, but relatively lower expression in roots and stems. With the extension of the treatment time, the expression of TvMYB1 in roots and stems has an increasing trend, but the opposite is true in leaves. Therefore, TvMYB1 is induced by osmotic stress, plays a role in T. vernicifluum stress response, and is an important candidate gene for research on the stress adaptation mechanism of T. vernicifluum.
keywords:Toxicodendron vernicifluum  MYB transcription factor  drought stress  qRT PCR
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